PA5-99147

antibody from Invitrogen Antibodies

Targeting: MAPKAPK5 MK5, PRAK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-99147

Provider

Invitrogen Antibodies

Product name

Phospho-PRAK (Thr182) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-PRAK (Thr182) in K562 cell lysate (left lane: treated with the antigen-specific peptide). Samples were incubated with Phospho-PRAK (Thr182) polyclonal antibody (Product # PA5-99147).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-PRAK (Thr182) in K562 cell lysate (left lane: treated with the antigen-specific peptide). Samples were incubated with Phospho-PRAK (Thr182) polyclonal antibody (Product # PA5-99147).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Phospho-PRAK (Thr182) in K562. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with Phospho-PRAK (Thr182) polyclonal antibody (Product # PA5-99147) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of paraffin-embedded Phospho-PRAK (Thr182) in human kidney tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-PRAK (Thr182) polyclonal antibody (Product # PA5-99147) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.