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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [4]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-22132 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- JMJD6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, NIH-3T3. Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (87%), Xenopus laevis (87%), Chicken (96%), Chimpanzee (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PSR using 30 µg of A431 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a PSR polyclonal antibody (Product # PA5-22132) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PSR using 30 µg of NIH-3T3 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a PSR polyclonal antibody (Product # PA5-22132) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of JMJD6 was performed by separating 30 µg of 293T lysates by 7.5% SDS PAGE. Proteins were transferred to a membrane and probed with a JMJD6 Polyclonal Antibody (Product # PA5-22132) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of JMJD6 was performed by separating 30 µg of NIH-3T3 lysates by 7.5% SDS PAGE. Proteins were transferred to a membrane and probed with a JMJD6 Polyclonal Antibody (Product # PA5-22132) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using PSR (Product # PA5-22132) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
