PA5-65442

antibody from Invitrogen Antibodies

Targeting: RELN PRO1598, RL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-65442

Provider

Invitrogen Antibodies

Product name

Reelin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: TFCEPYGPREL ITTGLNTTTA SVLQFSIGSG SCRFSYSDPS IIVLYAKNNS ADWIQLEKIR APSNVSTIIH ILYLPEDAKG ENVQFQWKQE NLRVGEVYE

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot was performed using Anti-Reelin Polyclonal Antibody (Product # PA5-65442) and a 400 kDa band corresponding to Reelin was observed across Hep G2 treated with PTI when compared to HCT 116. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Hep G2 treated with PTI (1X, for 4 hrs) (Lane 2), HCT 116 (Lane 3), HCT 116 treated with PTI (1X, for 4 hrs) (Lane 4) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:6000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Few uncharacterized bands were observed between 50-70 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of Reelin in human cell line U-2 OS shows localization to focal adhesion sites. Samples were probed using a Reelin Polyclonal Antibody (Product # PA5-65442).