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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
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Validation data
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- Product number
- MA1-23321 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RFC4 Monoclonal Antibody (1320)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1320
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of RFC2 in (A) HepG2, (B) LNCAP, (C) MCF7), (D) and SKOV3 whole cell lysates using a RFC2 monoclonal antibody (Product # MA1-23321).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RFC4 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a RFC4 Monoclonal Antibody (1320) (Product # MA1-23321) at a dilution of 1:500 and a HRP-conjugated anti-mouse IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RFC4 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a RFC4 Monoclonal Antibody (1320) (Product # MA1-23321) at a dilution of 1:500 and a HRP-conjugated anti-mouse IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RFC2 in methanol-fixed MCF7 cells using a RFC2 monoclonal antibody (Product # MA1-23321) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed MCF7, using p37 [1320] (Product # MA1-23321) antibody at 1:200 dilution.
