PA5-96340

antibody from Invitrogen Antibodies

Targeting: RFC4 A1, RFC37

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96340

Provider

Invitrogen Antibodies

Product name

RFC4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: DKIQQQRLLD IAKKENVKIS DEGIAYLVKV SEGDLRKAIT FLQSATRLTG GKEITEKVIT DIAGVIPAEK IDGVFAACQS GSFDKLEAVV KDLIDEGHAA TQLVNQLHDV VVENNLSDKQ KSIITEKLAE VDKCLADGAD EHLQLISLCA TVMQQLSQNC; Positive Samples: HeLa, SW480, 22Rv1, SW620, K-562, HepG2, MCF7; Cellular Location: Nucleus

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.69 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RFC4 in extracts of various cell lines using RFC4 Polyclonal Antibody (Product # PA5-96340) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RFC4 in extracts of various cell lines using RFC4 Polyclonal Antibody (Product # PA5-96340) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of RFC4 was performed in U2OS cells using RFC4 Polyclonal Antibody (Product # PA5-96340).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of RFC4 was performed in U2OS cells using RFC4 Polyclonal Antibody (Product # PA5-96340).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RFC4 in paraffin-embedded rat testis using RFC4 Polyclonal Antibody (Product # PA5-96340) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RFC4 in paraffin-embedded rat testis using RFC4 Polyclonal Antibody (Product # PA5-96340) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RFC4 in paraffin-embedded human breast cancer using RFC4 Polyclonal Antibody (Product # PA5-96340) at a dilution of 1:100.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of RFC4 was performed in 200 µg extracts of K562 cells using RFC4 Polyclonal Antibody (Product # PA5-96340). Western blot was performed from the immunoprecipitate using RFC4 Polyclonal Antibody.