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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-21538 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RFC4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse liver.
- Concentration
- 0.62 mg/mL
Submitted references Genome-wide RNAi Screening Identifies RFC4 as a Factor That Mediates Radioresistance in Colorectal Cancer by Facilitating Nonhomologous End Joining Repair.
Wang XC, Yue X, Zhang RX, Liu TY, Pan ZZ, Yang MJ, Lu ZH, Wang ZY, Peng JH, Le LY, Wang GY, Peng QH, Meng Y, Huang W, Liu RY
Clinical cancer research : an official journal of the American Association for Cancer Research 2019 Jul 15;25(14):4567-4579
Clinical cancer research : an official journal of the American Association for Cancer Research 2019 Jul 15;25(14):4567-4579
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RFC4 using 30 µg of A) H1299 (B) HeLa and C) HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a RFC4 polyclonal antibody (Product # PA5-21538) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RFC4 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a RFC4 Polyclonal Antibody (Product # PA5-21538) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RFC4 Polyclonal Antibody (Product # PA5-21538). Sample (50 µg of whole cell lysate). Lane A: mouse liver. 10% SDS PAGE. RFC4 Polyclonal Antibody (Product # PA5-21538) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RFC4 in paraformaldehyde-fixed A549 cells using a RFC4 polyclonal antibody (Product # PA5-21538) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed A549, using RFC4 (Product # PA5-21538) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of RFC4 was performed in paraffin-embedded MDA-MB-231xenograft tissue using RFC4 Polyclonal Antibody (Product # PA5-21538) at a dilution of 1:250.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded MDAMB-468 Xenograft, using RFC4 (Product # PA5-21538) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
