Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- NB100-234 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-234, RRID:AB_2178594
- Product name
- Goat Polyclonal RFC4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Evasion of early cellular response mechanisms following low level radiation-induced DNA damage.
Collis SJ, Schwaninger JM, Ntambi AJ, Keller TW, Nelson WG, Dillehay LE, Deweese TL
The Journal of biological chemistry 2004 Nov 26;279(48):49624-32
The Journal of biological chemistry 2004 Nov 26;279(48):49624-32
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RFC4 Antibody [NB100-234] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-RFC4 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RFC4 Antibody [NB100-234] - Whole cell extract (50 ug) from MCF7 and 293T or nuclear extract (50 ug) from HeLa cells. Antibody used at 0.5 ug/ml (A) and 1 ug/ml (B).