14326-1-AP

antibody from Proteintech Group

Targeting: RHOB ARH6, ARHB, MST081, RHOH6

Provider product page for 14326-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [33]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 14326-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#14326-1-AP, RRID:AB_2179092
Product name: RHOB antibody
Antibody type: Polyclonal
Description: KD/KO validated RHOB antibody (Cat. #14326-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

RHOB protein structure - 14326-1-AP shown in red.

Submitted references Electromagnetic exposure changes human Schwann cell motility and transcriptomic profile of hearing-loss-related genes.

Mohamed T, Colciago A, Faroni A, Reid AJ, Ferrero G, Magnaghi V
iScience 2026 Mar 20;29(3):115130

Phosphatidylserine and RhoB connect phosphatidylinositol 4-phosphate and phosphatidic acid metabolism at the plasma membrane.

Huang S, Kim YJ, Cao X, Bumpus TW, Sohn M, Menold MT, Dale RS, Kang JJ, Uematsu S, Gupta S, Qian SB, Yu H, Balla T, Baskin JM
bioRxiv : the preprint server for biology 2025 Oct 2;

Epigenetic ALYREF/UHRF1/RHOB Axis in Corneal Wound Healing and Implications for Epithelial Tumorigenesis.

Zhang H, Lan S, Ren D, Chen X, Lin Y, Cao Q, Xu W, Wang J, Sol Reinach P, Yan D, Luo G
Investigative ophthalmology & visual science 2025 Mar 3;66(3):54

Adducin-1 Facilitates Influenza Virus Endosomal Trafficking and Uncoating by Regulating Branched Actin Dynamics and Myosin IIB Activity.

Jiang M, Zou J, Jin Y, Jiang C, Tu S, Chen T, Guo J, Cheng Y, Jin M, Chen H, Zhou H
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Jul;12(28):e2417318

Engineered Exosomes Delivering bta-miR-223 Alleviate Staphylococcus aureus-Induced Mastitis in Mice via the TLR4/NF-κB Signaling Pathway.

Wang Y, Ouyang K, Nie Y, Yang J, Liao Y, Chen X, Wang H, Luo J, Sun J, Xi Q, Shen B, Chen T, Zhang Y
Journal of agricultural and food chemistry 2025 Jul 16;73(28):17824-17832

RhoB regulates prostate cancer cell proliferation and docetaxel sensitivity via the PI3K-AKT signaling pathway.

Sheng T, Su H, Yao L, Qu Z, Liu H, Shao W, Zhang X
BMC cancer 2025 Feb 26;25(1):354

The RhoB p.S73F mutation leads to cerebral palsy through dysregulation of lipid homeostasis.

Wu X, Liu R, Zhang Z, Yang J, Liu X, Jiang L, Fang M, Wang S, Lai L, Song Y, Li Z
EMBO molecular medicine 2024 Sep;16(9):2002-2023

AAMP and MTSS1 Are Novel Negative Regulators of Endothelial Barrier Function Identified in a Proteomics Screen.

Podieh F, Overboom MC, Knol JC, Piersma SR, Goeij-de Haas R, Pham TV, Jimenez CR, Hordijk PL
Cells 2024 Sep 25;13(19)

Downregulation of RhoB Inhibits Cervical Cancer Progression and Enhances Cisplatin Sensitivity.

Wang W, Jia Y, Liu Y, Lv X, Guo L, Meng S, Wang C
Genes 2024 Sep 10;15(9)

USP33 facilitates the ovarian cancer progression via deubiquitinating and stabilizing CBX2.

Chen J, Shan W, Jia Q, Chen Y, Jiang W, Tian Y, Huang X, Li X, Wang Z, Xia B
Oncogene 2024 Oct;43(43):3170-3183

Fluvastatin prevents lung metastasis in triple-negative breast cancer by triggering autophagy via the RhoB/PI3K/mTOR pathway.

Xu WH, Zhang T, Zhou Y, Mao Y
Experimental cell research 2024 Feb 1;435(1):113893

Transcriptomic analysis reveals potential crosstalk genes and immune relationship between triple-negative breast cancer and depression.

Zhuo Z, Lu W, Zhang L, Zhang D, Cui Y, Wu X, Mei H, Chang L, Song Q
Discover oncology 2024 Dec 18;15(1):762

Hepatocellular Carcinoma Cell-Derived Exosomal miR-21-5p Induces Macrophage M2 Polarization by Targeting RhoB.

Yu H, Pan J, Zheng S, Cai D, Luo A, Xia Z, Huang J
International journal of molecular sciences 2023 Feb 27;24(5)

MiR-21 Is Induced by Hypoxia and Down-Regulates RHOB in Prostate Cancer.

Angel CZ, Stafford MYC, McNally CJ, Nesbitt H, McKenna DJ
Cancers 2023 Feb 17;15(4)

Septin11 promotes hepatocellular carcinoma cell motility by activating RhoA to regulate cytoskeleton and cell adhesion.

Fu L, Wang X, Yang Y, Chen M, Kuerban A, Liu H, Dong Y, Cai Q, Ma M, Wu X
Cell death & disease 2023 Apr 20;14(4):280

Hsa-miR-3178/RhoB/PI3K/Akt, a novel signaling pathway regulates ABC transporters to reverse gemcitabine resistance in pancreatic cancer.

Gu J, Huang W, Wang X, Zhang J, Tao T, Zheng Y, Liu S, Yang J, Chen ZS, Cai CY, Li J, Wang H, Fan Y
Molecular cancer 2022 May 10;21(1):112

Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis.

Peng T, He Y, Wang T, Yu J, Ma X, Zhou Z, Sheng Y, Li L, Peng H, Li S, Zou J, Yuan Y, Zhao Y, Shi H, Li F, Liu W, Hu K, Lu X, Zhang G, Wang F
Journal of medicinal chemistry 2022 Aug 25;65(16):11010-11033

CUX1 Facilitates the Development of Oncogenic Properties Via Activating Wnt/β-Catenin Signaling Pathway in Glioma.

Feng F, Zhao Z, Zhou Y, Cheng Y, Wu X, Heng X
Frontiers in molecular biosciences 2021;8:705008

The association of transcription factor Prox1 with the proliferation, migration, and invasion of lung cancer.

Hao X, Luo W, Qiu X
Open life sciences 2021;16(1):602-610

GTPBP2 positively regulates the invasion, migration and proliferation of non-small cell lung cancer.

Jie L, Cong L, Conghui W, Ying G
Journal of Cancer 2021;12(13):3819-3826

A Complete Survey of RhoGDI Targets Reveals Novel Interactions with Atypical Small GTPases.

Ahmad Mokhtar AMB, Ahmed SBM, Darling NJ, Harris M, Mott HR, Owen D
Biochemistry 2021 May 18;60(19):1533-1551

Androgen receptor activation decreases proliferation in thyroid cancer cells.

Jones ME, O'Connell TJ, Zhao H, Darzynkiewicz Z, Gupta A, Buchsbaum J, Shin E, Iacob C, Suslina N, Moscatello A, Schantz S, Tiwari R, Geliebter J
Journal of cellular biochemistry 2021 Apr 20;

TMED3 Promotes Proliferation and Migration in Breast Cancer Cells by Activating Wnt/β-Catenin Signaling.

Zhang X, Luo Y, Li Q
OncoTargets and therapy 2020;13:5819-5830

Proteomic Profiling of Small Extracellular Vesicles Secreted by Human Pancreatic Cancer Cells Implicated in Cellular Transformation.

Servage KA, Stefanius K, Gray HF, Orth K
Scientific reports 2020 May 7;10(1):7713

Effects of mevalonate kinase interference on cell differentiation, apoptosis, prenylation and geranylgeranylation of human keratinocytes are attenuated by farnesyl pyrophosphate or geranylgeranyl pyrophosphate.

Li M, Min W, Wang J, Wang L, Li Y, Zhou N, Yang Z, Qian Q
Experimental and therapeutic medicine 2020 Apr;19(4):2861-2870

RALY may cause an aggressive biological behavior and a dismal prognosis in non-small-cell lung cancer.

Song G, Guo G, Du T, Li X, Wang J, Yan Y, Zhao Y
Experimental cell research 2020 Apr 15;389(2):111884

Atorvastatin Inhibits Breast Cancer Cells by Downregulating PTEN/AKT Pathway via Promoting Ras Homolog Family Member B (RhoB).

Ma Q, Gao Y, Xu P, Li K, Xu X, Gao J, Qi Y, Xu J, Yang Y, Song W, He X, Liu S, Yuan X, Yin W, He Y, Pan W, Wei L, Zhang J
BioMed research international 2019;2019:3235021

RETRACTED: MicroRNA-19a and microRNA-19b promote the malignancy of clear cell renal cell carcinoma through targeting the tumor suppressor RhoB.

Niu S, Ma X, Zhang Y, Liu YN, Chen X, Gong H, Yao Y, Liu K, Zhang X
PloS one 2018;13(2):e0192790

ATR/Chk1 signaling induces autophagy through sumoylated RhoB-mediated lysosomal translocation of TSC2 after DNA damage.

Liu M, Zeng T, Zhang X, Liu C, Wu Z, Yao L, Xie C, Xia H, Lin Q, Xie L, Zhou D, Deng X, Chan HL, Zhao TJ, Wang HR
Nature communications 2018 Oct 8;9(1):4139

RETRACTED: RhoB Acts as a Tumor Suppressor That Inhibits Malignancy of Clear Cell Renal Cell Carcinoma.

Chen W, Niu S, Ma X, Zhang P, Gao Y, Fan Y, Pang H, Gong H, Shen D, Gu L, Zhang Y, Zhang X
PloS one 2016;11(7):e0157599

MicroRNA-223 Attenuates Hypoxia-induced Vascular Remodeling by Targeting RhoB/MLC2 in Pulmonary Arterial Smooth Muscle Cells.

Zeng Y, Zhang X, Kang K, Chen J, Wu Z, Huang J, Lu W, Chen Y, Zhang J, Wang Z, Zhai Y, Qu J, Ramchandran R, Raj JU, Wang J, Gou D
Scientific reports 2016 Apr 28;6:24900

Overexpression of RNF146 in non-small cell lung cancer enhances proliferation and invasion of tumors through the Wnt/β-catenin signaling pathway.

Gao Y, Song C, Hui L, Li CY, Wang J, Tian Y, Han X, Chen Y, Tian DL, Qiu X, Wang E
PloS one 2014;9(1):e85377

Expression signatures of the lipid-based Akt inhibitors phosphatidylinositol ether lipid analogues in NSCLC cells.

Zhang C, Elkahloun AG, Liao H, Delaney S, Saber B, Morrow B, Prendergast GC, Hollander MC, Gills JJ, Dennis PA
Molecular cancer therapeutics 2011 Jul;10(7):1137-48

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse brain tissue were subjected to SDS PAGE followed by western blot with 14326-1-AP(RHOB antibody) at dilution of 1:2000
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of MCF-7 cells, using RHOB antibody 14326-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line