MA5-17032

GDIA1, RHOGDI

Antibody data

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Validation data

Product number: MA5-17032
Provider: Invitrogen Antibodies
Product name: Anti-RhoGDI Monoclonal Antibody (2G3)
Provider product page: Invitrogen Antibodies - MA5-17032
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: MA5-17032 targets ARHGDIA in FACS, ICC, IF and WB applications and shows reactivity with Human, Mouse, and Non-human primate samples. The MA5-17032 immunogen is purified recombinant fragment of human ARHGDIA (amino acids: FULL(1-204)) expressed in E. Coli. MA5-17032 detects ARHGDIA which has a predicted molecular weight of approximately 26kDa.
Reactivity: Human, Mouse, Rat
Host: Mouse
Isotype: IgG
Antibody clone number: 2G3
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Provider	Type	Product Number
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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ARHGDIA using a ARHGDIA monoclonal antibody (Product # MA5-17032) against a human ARHGDIA recombinant protein.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ARHGDIA using ARHGDIA monoclonal antibody (Product # MA5-17032) in Jurkat (1), HeLa (2), NIH3T3 (3), C6 (4), K562 (5), and COS-7 (6) cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of RhoGDI was achieved by transfecting HeLa with RhoGDI specific siRNAs (Silencer® select Product # s698, s699). Western blot analysis (Fig. a) was performed using Whole Cell Extract-WCL from the RhoGDI knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with RhoGDI Monoclonal Antibody (2G3) (Product # MA5-17032, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to RhoGDI.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-RhoGDI Monoclonal Antibody (2G3)(Product # MA5-17032) and a 25kDa band corresponding to RhoGDI was observed across the cell lines tested. Whole Cell Extract-WCL (30 ug µg lysate) of SH-SY5Y (Lane 1), U-937 (Lane 2), NIH:OVCAR-3 (Lane 3), COS-7 (Lane 4), NIH/3T3 (Lane 5), HeLa (Lane 6) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).