TA501089

antibody from OriGene

Targeting: ARHGDIA GDIA1, RHOGDI

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501089

Provider

OriGene

Proper citation

OriGene Cat#TA501089, RRID:AB_11125188

Product name

ARHGDIA (RhoGDI) mouse monoclonal antibody, clone OTI1F2 (formerly 1F2)

Antibody type

Monoclonal

Description

ARHGDIA (RhoGDI) mouse monoclonal antibody, clone OTI1F2 (formerly 1F2)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

ARHGDIA

Isotype

IgG

Antibody clone number

OTI1F2

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARHGDIA monoclonal antibody.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARHGDIA (RC200902, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHGDIA.

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC200902 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ARHGDIA antibody(TA501089), and then analyzed by flow cytometry.

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Hela cells, using anti-ARHGDIA antibody(TA501089),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

Validation comment

FC