sc-46659

antibody from Santa Cruz Biotechnology

Targeting: RXRA NR2B1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-46659

Provider

Santa Cruz Biotechnology

Proper citation

Product name

Anti-RXRA

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

References

Submitted references

A novel bile acid-activated vitamin D receptor signaling in human hepatocytes.

Han S, Li T, Ellis E, Strom S, Chiang JY
Molecular endocrinology (Baltimore, Md.) 2010 Jun;24(6):1151-64

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Retinoic acid activates monoamine oxidase B promoter in human neuronal cells.

Wu JB, Chen K, Ou XM, Shih JC
The Journal of biological chemistry 2009 Jun 19;284(25):16723-16735

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Retinoic acid induces prostatic bud formation.

Vezina CM, Allgeier SH, Fritz WA, Moore RW, Strerath M, Bushman W, Peterson RE
Developmental dynamics : an official publication of the American Association of Anatomists 2008 May;237(5):1321-33

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Chaperone-dependent stabilization and degradation of p53 mutants

P Muller, R Hrstka, D Coomber, D P Lane, B Vojtesek
Oncogene 2008 Jan;27(24):3371-3383

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

No bands detected.

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 51

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells and lymphoid cells outside reaction centra.

Validation comment

Staining pattern partly consistent with experimental and/or bioinformatic data.