Antibody data
A novel bile acid-activated vitamin D receptor signaling in human hepatocytes.
Han S, Li T, Ellis E, Strom S, Chiang JY
Molecular endocrinology (Baltimore, Md.) 2010 Jun;24(6):1151-64
Retinoic acid activates monoamine oxidase B promoter in human neuronal cells.
Wu JB, Chen K, Ou XM, Shih JC
The Journal of biological chemistry 2009 Jun 19;284(25):16723-35
Retinoic acid induces prostatic bud formation.
Vezina CM, Allgeier SH, Fritz WA, Moore RW, Strerath M, Bushman W, Peterson RE
Developmental dynamics : an official publication of the American Association of Anatomists 2008 May;237(5):1321-33
Chaperone-dependent stabilization and degradation of p53 mutants
P Muller, R Hrstka, D Coomber, D P Lane, B Vojtesek
Oncogene 2008 Jan;27(24):3371-3383
Supportive validation
- Submitted by
-
per
- Main image

- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- No bands detected.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 51
Supportive validation
- Submitted by
-
per
- Main image

- Experimental details
- Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells and lymphoid cells outside reaction centra.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.