21218-1-AP

antibody from Proteintech Group

Targeting: RXRA NR2B1

Provider product page for 21218-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [58]
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Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [1]

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Product number: 21218-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#21218-1-AP, RRID:AB_10693633
Product name: RXRA antibody
Antibody type: Polyclonal
Description: KD/KO validated RXRA antibody (Cat. #21218-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

RXRA protein structure - 21218-1-AP shown in red.

Submitted references TEAD4 and RXRA Regulate the Function of Nucleus Pulposus Cells in Intervertebral Disc Degeneration Via the TNF-α/NF-κB Pathway: An Integrated Analysis of Single-Cell RNA-Seq, Bulk RNA-Seq, and In Vitro Validation.

Yang L, Liu H, Pu J, Hu Y, Deng Y, Yan X, Liu M
Applied biochemistry and biotechnology 2026 Mar;198(3):1939-1961

Xiaoyaosan ameliorates stress-induced MASH via modulating IL-1R1/RXRα-mediated liver-brain crosstalk.

Zhang Y, Luo X, Wu S, Qin Y, Xue Q, Ye W, Hou S, Liu B, Huang D, Yuan W, Ao H, Tong G, Liao M
Journal of ethnopharmacology 2026 Jun 28;365:121585

Babaodan alleviates MAFLD through hepatic glycerophospholipid metabolism and PPARγ/RXRA/GPAT3 based on spatial metabolomics and proteomics analysis.

Qiu S, Lai Z, Wang Z, Zeng J, Guo S, Yang L, Xie D, Cai Y, Wang Y, Che L, Zhang A
Journal of ethnopharmacology 2026 Jun 12;364:121475

Structural and mechanistic analysis of covalent ligands targeting the RNA-binding protein NONO.

Lindsey GL, Hockley TK, Gomez AV, Marshall AC, Brothers WR, Finney CT, Gross J, Fox AH, Yeo GW, Melillo B, Bond CS, Cravatt BF
Cell chemical biology 2026 Feb 19;33(2):256-267.e11

Selenoprotein H Functions as a PPARα Coactivator to Link Selenium Homeostasis to Hepatic Lipid Metabolism and Protect against Steatohepatitis.

Zhang Y, Wang Y, Li B, Li X, Liu C, Chen Y, Tian C, Wang D, Gu X, Jiang C, Wei Y, Ding Q
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Apr;13(22):e19563

Genome-wide chromatin profiling reveals a non-limiting role for RXR in macrophage-like cells stimulated with multiple nuclear receptor agonists.

Mianesaz H, Göczi L, Bojcsuk D, Ghalamkari S, Fadel L, Póliska S, Penyige A, Nagy L, Nagy G, Vámosi G, Széles L
The Journal of biological chemistry 2026 Apr 22;:111486

Multi-omics analysis of the active components and mechanisms of Baojin Chenfei formula in silica-induced murine silicosis.

Wr H, Yq L, Xg L, Yf Z, Y W, P Z, Js L, Q Z
Phytomedicine : international journal of phytotherapy and phytopharmacology 2025 Sep;145:156986

Aerobic Exercise Attenuates Autophagy-Lysosomal Flux Deficits via β2-AR-Mediated ESCRT-III Subunit CHMP4B in Mice With Human MAPT P301L.

Bi SG, Yu H, Gao TL, Wu JJ, Mao YM, Gong J, Wang FZ, Yang L, Chen J, Lan ZC, Shen MT, Nie YJ, Chai GS
Aging cell 2025 Oct;24(10):e70184

Glutathione contributes to alleviating hepatic injury via host-microbiome interaction and CAR-dependent pathway.

Xiang X, Liu S, Wang H, Wang C, Zhou W, Chen H, Feng Y, He X, Xu G, Zhao Q, Li T, Liu D, Yin Y, He L
Science China. Life sciences 2025 Oct 10;

Activation of pregnane X receptor-organic anion transporting polypeptide 1a/b /P-glycoprotein/multidrug resistance protein 2 axis mediates the accelerated blood and liver clearance of PEGylated liposomes.

Pan J, Zhang C, Lu H, Zhang X, Deng H, Chen Y, Wang L, Zhang S, Wang F, Chen W
Drug metabolism and disposition: the biological fate of chemicals 2025 Nov;53(11):100172

Obacunone ameliorates high-fat diet-induced MAFLD by regulating the PPARγ-FABP1/CD36 axis and the gut-liver crosstalk.

Wang X, Hong J, Liang R, Bao S, Li L, Wang J, Feng J
Phytomedicine : international journal of phytotherapy and phytopharmacology 2025 Nov;147:157180

Sodium hexametaphosphate mitigates calcium oxalate stone formation by modulating crystallization and maintaining PPARα signaling.

Xian JY, Peng L, Long J, Chen XW, Huang LH, Sun XY
Urolithiasis 2025 Nov 18;53(1):222

Ursodeoxycholic acid alleviates aortic aneurysm and dissection through the intestinal farnesoid X receptor/ceramide synthase 2 axis.

Zhang Z, Xie L, Lin X, He J, Xie Y, Li J, Zhuang X, Tang L, Xie R, Wu Q, Qiu Z, Chen L
Communications biology 2025 Jul 5;8(1):1009

Discovery of PXR Antagonist MI891 and PXR Degrader MI1013 and Their Roles in Hepatic Gene Regulation.

Kamaraj R, Mejdrová I, Krutakova M, Smutny T, Škach K, Dohnalova K, Smutna L, Nellore DSS, Dusek J, Chalupsky K, Hricová J, Kronenberger T, Stahl A, Templin M, Braeuning A, Nencka R, Pavek P
Journal of medicinal chemistry 2025 Jul 24;68(14):14271-14299

Multi-omics analysis and experimental verification reveal testicular fatty acid metabolism disorder in non-obstructive azoospermia.

Li Z, Xiang YJ, Zou ZC, Feng YM, Wang H, Chen WQ, Ge X, Ma JZ, Jing J, Yao B
Zoological research 2025 Jan 18;46(1):177-192

Surprising features of nuclear receptor interaction networks revealed by live-cell single-molecule imaging.

Dahal L, Graham TGW, Dailey GM, Heckert A, Tjian R, Darzacq X
eLife 2025 Jan 10;12

Transcriptome Reveals the Promoting Effect of Beta-Sitosterol on the Differentiation of Bovine Preadipocytes.

Jiang L, Sun X, Wan Y, Qin Q, Xu M, Ma J, Zan L, Wang H
Journal of agricultural and food chemistry 2025 Feb 12;73(6):3400-3412

Magnolol preserves the integrity of the intestinal epithelial barrier and mitigates intestinal injury through activation of PPAR γ in COPD rat.

Tao L, Zhang Q, Liu L, Wang K, Liu X, Li J, Zhao P
Journal of ethnopharmacology 2025 Feb 10;338(Pt 2):119068

Surprising Features of Nuclear Receptor Interaction Networks Revealed by Live Cell Single Molecule Imaging.

Dahal L, Graham TG, Dailey GM, Heckert A, Tjian R, Darzacq X
bioRxiv : the preprint server for biology 2024 Oct 30;

The single-cell transcriptomic landscape of the topological differences in mammalian auditory receptors.

Ma X, Chen X, Che Y, Zhu S, Wang X, Gao S, Wu J, Kong F, Cheng C, Wu Y, Guo J, Qi J, Chai R
Science China. Life sciences 2024 Nov;67(11):2398-2410

Integrative analyses of genetic mechanisms responsible for bone-fat imbalance in osteoporosis.

Zhang Z, Tao Z, Zhang Y, Zhang Z, Zhang W, Zhang X, Zhao J, Tao C, Zhou X
The journal of gene medicine 2024 Nov;26(11):e3739

LncBNIP3 Inhibits Bovine Intramuscular Preadipocyte Differentiation via the PI3K-Akt and PPAR Signaling Pathways.

Zhang W, Raza SHA, Li B, Yang W, Khan R, Aloufi BH, Zhang G, Zuo F, Zan L
Journal of agricultural and food chemistry 2024 Nov 6;72(44):24260-24271

Zinc lactate alleviates oxidative stress by modulating crosstalk between constitutive androstane receptor signaling pathway and gut microbiota profile in weaned piglets.

Tang W, Xiang X, Wang H, Zhou W, He L, Yin Y, Li T
Animal nutrition (Zhongguo xu mu shou yi xue hui) 2024 Mar;16:23-33

Comparative efficacy of sweated and non-sweated Salvia miltiorrhiza Bge. extracts on acute myocardial ischemia via regulating the PPARα/RXRα/NF-κB signaling pathway.

Shan X, Li J, Hong B, Yin H, Lu Z, Wang G, Yu N, Peng D, Wang L, Zhang C, Chen W
Heliyon 2024 Jun 15;10(11):e31923

ZNF148 inhibits HBV replication by downregulating RXRα transcription.

Yao X, Xu K, Tao N, Cheng S, Chen H, Zhang D, Yang M, Tan M, Yu H, Chen P, Zhan Z, He S, Li R, Wang C, Wu D, Ren J
Virology journal 2024 Jan 31;21(1):35

Hammerhead-type FXR agonists induce an eRNA FincoR that ameliorates nonalcoholic steatohepatitis in mice.

Chen J, Wang R, Xiong F, Sun H, Kemper B, Li W, Kemper JK
bioRxiv : the preprint server for biology 2024 Feb 8;

Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke.

Guo H, Li Y, Wang S, Yang Y, Xu T, Zhao J, Wang J, Zuo W, Wang P, Zhao G, Wang H, Hou W, Dong H, Cai Y
Redox biology 2024 Aug;74:103234

Hammerhead-type FXR agonists induce an enhancer RNA Fincor that ameliorates nonalcoholic steatohepatitis in mice.

Chen J, Wang R, Xiong F, Sun H, Kemper B, Li W, Kemper J
eLife 2024 Apr 15;13

Modification of lysine-260 2-hydroxyisobutyrylation destabilizes ALDH1A1 expression to regulate bladder cancer progression.

Zhang Z, Wang Y, Liang Z, Meng Z, Zhang X, Ma G, Chen Y, Zhang M, Su Y, Li Z, Liang Y, Niu H
iScience 2023 Nov 17;26(11):108142

Alpha lipoamide inhibits diabetic kidney fibrosis via improving mitochondrial function and regulating RXRα expression and activation.

Zhang HF, Liu HM, Xiang JY, Zhou XC, Wang D, Chen RY, Tan WL, Liang LQ, Liu LL, Shi MJ, Zhang F, Xiao Y, Zhou YX, Zhang T, Tang L, Guo B, Wang YY
Acta pharmacologica Sinica 2023 May;44(5):1051-1065

γ-Linolenic acid in maternal milk drives cardiac metabolic maturation.

Paredes A, Justo-Méndez R, Jiménez-Blasco D, Núñez V, Calero I, Villalba-Orero M, Alegre-Martí A, Fischer T, Gradillas A, Sant'Anna VAR, Were F, Huang Z, Hernansanz-Agustín P, Contreras C, Martínez F, Camafeita E, Vázquez J, Ruiz-Cabello J, Area-Gómez E, Sánchez-Cabo F, Treuter E, Bolaños JP, Estébanez-Perpiñá E, Rupérez FJ, Barbas C, Enríquez JA, Ricote M
Nature 2023 Jun;618(7964):365-373

Neocryptotanshinone ameliorates insufficient energy production in heart failure by targeting retinoid X receptor alpha.

Ma L, Shao M, Cheng W, Jiang J, Chen X, Tan N, Ling G, Yang Y, Wang Q, Yang R, Li C, Wang Y
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023 Jul;163:114868

A Novel in Duck Myoblasts: The Transcription Factor Retinoid X Receptor Alpha (RXRA) Inhibits Lipid Accumulation by Promoting CD36 Expression.

Pan Z, Chen X, Wu D, Li X, Gao W, Li G, Du G, Zhang C, Jin S, Geng Z
International journal of molecular sciences 2023 Jan 7;24(2)

Downregulation of AC092894.1 promotes oxaliplatin resistance in colorectal cancer via the USP3/AR/RASGRP3 axis.

Zheng Z, Wu M, Li H, Xu W, Yang M, Pan K, Ni Y, Jiang T, Zheng H, Jin X, Zhang Y, Ding L, Fu J
BMC medicine 2023 Apr 3;21(1):132

Manganese-Induced Oxidative Stress Contributes to Intestinal Lipid Deposition via the Deacetylation of PPARγ at K339 by SIRT1.

Lv WH, Zhao T, Pantopoulos K, Chen GH, Wei XL, Zhang DG, Luo Z
Antioxidants & redox signaling 2022 Sep;37(7-9):417-436

Animal models of male subfertility targeted on LanCL1-regulated spermatogenic redox homeostasis.

Huang C, Yang C, Pang D, Li C, Gong H, Cao X, He X, Chen X, Mu B, Cui Y, Liu W, Luo Q, Cheng A, Jia L, Chen M, Xiao B, Chen Z
Lab animal 2022 May;51(5):133-145

Downregulation of lncRNA Miat contributes to the protective effect of electroacupuncture against myocardial fibrosis.

Qi W, Li X, Ren Y, Liu X, Fu H, Wang X, Li X, Xiong J, Zheng Q, Cai D, Liang F
Chinese medicine 2022 May 17;17(1):57

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Burda JE, O'Shea TM, Ao Y, Suresh KB, Wang S, Bernstein AM, Chandra A, Deverasetty S, Kawaguchi R, Kim JH, McCallum S, Rogers A, Wahane S, Sofroniew MV
Nature 2022 Jun;606(7914):557-564

Panaxytriol upregulates CYP3A4 expression based on the interaction of PXR, CAR, HSP90α, and RXRα.

Zhang L, Yan J, Liu J, Meng C, Liu F, Xia C
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Jul;101:154097

Integrated analysis of mRNA-seq and miRNA-seq reveals the potential roles of Egr1, Rxra and Max in kidney stone disease.

Huang L, Shi Y, Hu J, Ding J, Guo Z, Yu B
Urolithiasis 2022 Dec 9;51(1):13

Oscillating lncRNA Platr4 regulates NLRP3 inflammasome to ameliorate nonalcoholic steatohepatitis in mice.

Lin Y, Wang S, Gao L, Zhou Z, Yang Z, Lin J, Ren S, Xing H, Wu B
Theranostics 2021;11(1):426-444

GYY4137 Regulates Extracellular Matrix Turnover in the Diabetic Kidney by Modulating Retinoid X Receptor Signaling.

Juin SK, Pushpakumar S, Sen U
Biomolecules 2021 Oct 7;11(10)

Comprehensive characterization of protein-protein interactions perturbed by disease mutations.

Cheng F, Zhao J, Wang Y, Lu W, Liu Z, Zhou Y, Martin WR, Wang R, Huang J, Hao T, Yue H, Ma J, Hou Y, Castrillon JA, Fang J, Lathia JD, Keri RA, Lightstone FC, Antman EM, Rabadan R, Hill DE, Eng C, Vidal M, Loscalzo J
Nature genetics 2021 Mar;53(3):342-353

Caveolin-1 facilitates cell migration by upregulating nuclear receptor 4A2/retinoid X receptor α-mediated β-galactoside α2,6-sialyltransferase I expression in human hepatocarcinoma cells.

Chen X, Wang L, Yu X, Wang S, Zhang J
The international journal of biochemistry & cell biology 2021 Aug;137:106027

Heparin-binding growth factor (HDGF) drives radioresistance in breast cancer by activating the STAT3 signaling pathway.

Qiu L, Ma Y, Chen X, Zhou L, Zhang H, Zhong G, Zhang L, Tang J
Journal of translational medicine 2021 Aug 10;19(1):344

Bergenin Exerts Hepatoprotective Effects by Inhibiting the Release of Inflammatory Factors, Apoptosis and Autophagy via the PPAR-γ Pathway.

Xiang S, Chen K, Xu L, Wang T, Guo C
Drug design, development and therapy 2020;14:129-143

Inhaled vitamin A is more effective than intramuscular dosing in mitigating hyperoxia-induced lung injury in a neonatal rat model of bronchopulmonary dysplasia.

Gelfand CA, Sakurai R, Wang Y, Liu Y, Segal R, Rehan VK
American journal of physiology. Lung cellular and molecular physiology 2020 Sep 1;319(3):L576-L584

Small-molecule inhibitor targeting orphan nuclear receptor COUP-TFII for prostate cancer treatment.

Wang L, Cheng CM, Qin J, Xu M, Kao CY, Shi J, You E, Gong W, Rosa LP, Chase P, Scampavia L, Madoux F, Spicer T, Hodder P, Xu HE, Tsai SY, Tsai MJ
Science advances 2020 May;6(18):eaaz8031

High-Dose Dexamethasone Manipulates the Tumor Microenvironment and Internal Metabolic Pathways in Anti-Tumor Progression.

Xu L, Xia H, Ni D, Hu Y, Liu J, Qin Y, Zhou Q, Yi Q, Xie Y
International journal of molecular sciences 2020 Mar 7;21(5)

Peroxisome proliferator-activated receptor-γ coactivator 1α-mediated pathway as a possible therapeutic target in endometriosis.

Kataoka H, Mori T, Okimura H, Matsushima H, Ito F, Koshiba A, Tanaka Y, Akiyama K, Maeda E, Sugahara T, Tarumi Y, Kusuki I, Khan KN, Kitawaki J
Human reproduction (Oxford, England) 2019 Jun 4;34(6):1019-1029

Activation of Pregnane X Receptor-Cytochrome P450s Axis: A Possible Reason for the Enhanced Accelerated Blood Clearance Phenomenon of PEGylated Liposomes In Vivo.

Wang F, Wang H, Wu Y, Wang L, Zhang L, Ye X, Peng D, Chen W
Drug metabolism and disposition: the biological fate of chemicals 2019 Aug;47(8):785-793

Bexarotene protects against neurotoxicity partially through a PPARγ-dependent mechanism in mice following traumatic brain injury.

He J, Liu H, Zhong J, Guo Z, Wu J, Zhang H, Huang Z, Jiang L, Li H, Zhang Z, Liu L, Wu Y, Qi L, Sun X, Cheng C
Neurobiology of disease 2018 Sep;117:114-124

Prevention of Oxidative Stress by α-Ketoglutarate via Activation of CAR Signaling and Modulation of the Expression of Key Antioxidant-Associated Targets in Vivo and in Vitro.

He L, Wu J, Tang W, Zhou X, Lin Q, Luo F, Yin Y, Li T
Journal of agricultural and food chemistry 2018 Oct 31;66(43):11273-11283

LncRNA DANCR upregulates PI3K/AKT signaling through activating serine phosphorylation of RXRA.

Tang J, Zhong G, Zhang H, Yu B, Wei F, Luo L, Kang Y, Wu J, Jiang J, Li Y, Wu S, Jia Y, Liang X, Bi A
Cell death & disease 2018 Dec 5;9(12):1167

Nrf2 activation is required for curcumin to induce lipocyte phenotype in hepatic stellate cells.

Lu C, Xu W, Zheng S
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2017 Nov;95:1-10

Nrf2 induces lipocyte phenotype via a SOCS3-dependent negative feedback loop on JAK2/STAT3 signaling in hepatic stellate cells.

Lu C, Xu W, Shao J, Zhang F, Chen A, Zheng S
International immunopharmacology 2017 Aug;49:203-211

Long noncoding RNA HULC modulates abnormal lipid metabolism in hepatoma cells through an miR-9-mediated RXRA signaling pathway.

Cui M, Xiao Z, Wang Y, Zheng M, Song T, Cai X, Sun B, Ye L, Zhang X
Cancer research 2015 Mar 1;75(5):846-57

Activation of RAS/ERK alone is insufficient to inhibit RXRα function and deplete retinoic acid in hepatocytes.

Wang AG, Song YN, Chen J, Li HL, Dong JY, Cui HP, Yao L, Li XF, Gao WT, Qiu ZW, Wang FJ, Wang JY
Biochemical and biophysical research communications 2014 Sep 26;452(3):801-7

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: MCF7 cells were subjected to SDS PAGE followed by western blot with 21218-1-AP(RXRA antibody) at dilution of 1:600
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using RXRA antibody 21218-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human kidney using 21218-1-AP(RXRA antibody) at dilution of 1:200 (under 10x lens)
Sample type: tissue