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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- RQ4236 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- RXRA Antibody / Retinoic X Receptor alpha
- Antibody type
- Polyclonal
- Description
- This highly specific RXRA antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Immunocytochemistry/Flow cytometry/ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the RXRA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa, 2) COLO320, 3) A431, 4) MCF7, 5) rat heart and 6) mouse heart lysate with RXRA antibody at 0.5ug/ml. Predicted molecular weight: ~51 kDa but routinely observed at 54~60 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE mouse liver tissue with RXRA antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE rat liver tissue with RXRA antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
