Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- Ab125125 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant RXRA Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human Retinoid X Receptor alpha/RXRA Antibody, Recombinant, could be used for WB, ICC, IF and so on.ApplicationWB: 1/500-1/1000ICC/IF: 1/50-1/200Protein Function Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 1-14
- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,5 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Retinoid X Receptor alpha/RXRA Antibody (Ab125125) - Western Blot All lanes: Recombinant Retinoid X Receptor alpha/RXRA Antibody (Ab125125) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 51 kDa Observed band size: 54 kDa Exposure time: 52.9 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Retinoid X Receptor alpha/RXRA Antibody (Ab125125) - IF/ICC Immunocytochemistry analysis of Retinoid X Receptor alpha (green) in MCF7 using Recombinant Retinoid X Receptor alpha/RXRA Antibody (Ab125125), and cells were counterstained with DAPI (blue).