Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-1466 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1466, RRID:AB_2184868
- Product name
- Goat Polyclonal RXR alpha/NR2B1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise an epitope corresponding to aa 14-28 of human RXR alpha protein and does not cross-react with either RXR beta or gamma.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Flow cytometry-based FRET identifies binding intensities in PPARγ1 protein-protein interactions in living cells.
Reduction of atherosclerosis in apolipoprotein E knockout mice by activation of the retinoid X receptor.
Trümper V, von Knethen A, Preuß A, Ermilov E, Hackbarth S, Kuchler L, Gunne S, Schäfer A, Bornhütter T, Vereb G, Ujlaky-Nagy L, Brüne B, Röder B, Schindler M, Parnham MJ, Knape T
Theranostics 2019;9(19):5444-5463
Theranostics 2019;9(19):5444-5463
Reduction of atherosclerosis in apolipoprotein E knockout mice by activation of the retinoid X receptor.
Claudel T, Leibowitz MD, Fiévet C, Tailleux A, Wagner B, Repa JJ, Torpier G, Lobaccaro JM, Paterniti JR, Mangelsdorf DJ, Heyman RA, Auwerx J
Proceedings of the National Academy of Sciences of the United States of America 2001 Feb 27;98(5):2610-5
Proceedings of the National Academy of Sciences of the United States of America 2001 Feb 27;98(5):2610-5
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RXR alpha/NR2B1 Antibody [NB100-1466] - Nuclear HeLa lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RXR alpha/NR2B1 Antibody [NB100-1466] - Western blot analysis of nuclear HeLa cell lysate (A) + peptide (B) and nuclear K562 cell lysate (C) + peptide (D). 35 ug protein in RIPA buffer. Antibody at 0.3 ug/mL. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: RXR alpha/NR2B1 Antibody [NB100-1466] - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.