11947-1-AP

antibody from Proteintech Group

Targeting: RAB5A RAB5

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

11947-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#11947-1-AP, RRID:AB_2269388

Product name

RAB5A antibody

Antibody type

Polyclonal

Description

KD/KO validated RAB5A antibody (Cat. #11947-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

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Retroviral foamy virus gag induces parkin-dependent mitophagy.

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Ginsenoside 1 mitigates postoperative cognitive dysfunction by enhancing microglial Aβ clearance through the endo-lysosomal pathway.

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Cholesterol-dependent Nsp5-endosomes co-trafficking to lysosomes facilitates porcine reproductive and respiratory syndrome virus replication by activating autophagy.

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PI3K/mTOR Inhibitor Induces Context-Dependent Apoptosis and Methuosis in Cancer Cells.

Hua X, Chen P, Zeng W, Han Y, Guo Y, Chen Y, Li C, Du Y, Ma M, Dong S
Pharmaceuticals (Basel, Switzerland) 2025 Dec 4;18(12)

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The involvement of Rab5 in regulating haematopoiesis in the Chinese mitten crab Eriocheir sinensis.

Qiao X, Zhao X, Zeng Y, Gu X, Wang Y, Yu H, He M, Wang L, Song L
Fish & shellfish immunology 2025 Aug;163:110363

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Caveolin Regulates the Transport Mechanism of the Walnut-Derived Peptide EVSGPGYSPN to Penetrate the Blood-Brain Barrier.

Li Z, Dang Q, Liu C, Liu Y, Wang C, Zhao F, Wang Q, Min W
Journal of agricultural and food chemistry 2024 Sep 11;72(36):19786-19799

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PTP1B Modulates Carotid Plaque Vulnerability in Atherosclerosis Through Rab5-PDGFRβ-Mediated Endocytosis Disruption and Apoptosis.

Zhang X, Xu R, Wang T, Li J, Sun Y, Cui S, Xing Z, Lyu X, Yang G, Jiao L, Li W
CNS neuroscience & therapeutics 2024 Nov;30(11):e70071

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Sensing steroid hormone 17α-hydroxypregnenolone by GPR56 enables protection from ferroptosis-induced liver injury.

Lin H, Ma C, Zhuang X, Liu S, Liu D, Zhang M, Lu Y, Zhou G, Zhang C, Wang T, Zhang Z, Lv L, Zhang D, Ruan XZ, Xu Y, Chai R, Yu X, Sun JP, Chu B
Cell metabolism 2024 Nov 5;36(11):2402-2418.e10

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TMED2 promotes glioma tumorigenesis by being involved in EGFR recycling transport.

Sun C, Zhang Y, Wang Z, Chen J, Zhang J, Gu Y
International journal of biological macromolecules 2024 Mar;262(Pt 2):130055

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Cell death induced by nicotine in human neuroblastoma SH-SY5Y cells is mainly attributed to cytoplasmic vacuolation originating from the trans-Golgi network.

Li J, Wang H, Chen H, Li X, Liu Y, Hou H, Hu Q
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2024 Mar;185:114431

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Propofol improves survival in a murine model of sepsis via inhibiting Rab5a-mediated intracellular trafficking of TLR4.

Zhou BW, Zhang WJ, Zhang FL, Yang X, Ding YQ, Yao ZW, Yan ZZ, Zhao BC, Chen XD, Li C, Liu KX
Journal of translational medicine 2024 Mar 28;22(1):316

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miR-451a suppresses the proliferation and migration of high-grade serous ovarian cancer by targeting RAB5A through the Ras/Raf/MEK/ERK pathway.

Liu S, Wang K, Zhao Z, Pang Y, Liu F, Wang P, Wang Z, Yang X
The journal of gene medicine 2024 Jan;26(1):e3649

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trans-Endothelial neutrophil migration activates bactericidal function via Piezo1 mechanosensing.

Mukhopadhyay A, Tsukasaki Y, Chan WC, Le JP, Kwok ML, Zhou J, Natarajan V, Mostafazadeh N, Maienschein-Cline M, Papautsky I, Tiruppathi C, Peng Z, Rehman J, Ganesh B, Komarova Y, Malik AB
Immunity 2024 Jan 9;57(1):52-67.e10

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Molecular basis for the different PCV2 susceptibility of T-lymphoblasts in Landrace and Piétrain pigs.

Ouyang Y, Nauwynck HJ
Veterinary research 2024 Feb 19;55(1):22

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Absence of motor impairments or pathological changes in TMEM230 knockout rats.

Zhang W, Peng H, Yang D, Song G, He J, Zhou Y, Huang C, Huang B
Neuroscience letters 2024 Aug 10;837:137921

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Targeting GDP-Dissociation Inhibitor Beta (GDI2) with a Benzo[a]quinolizidine Library to Induce Paraptosis for Cancer Therapy.

Sun Y, Zheng H, Qian L, Liu Y, Zhu D, Xu Z, Chang W, Xu J, Wang L, Sun B, Gu L, Yuan H, Lou H
JACS Au 2023 Oct 23;3(10):2749-2762

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Targeting intracellular oncoproteins with dimeric IgA promotes expulsion from the cytoplasm and immune-mediated control of epithelial cancers.

Biswas S, Mandal G, Anadon CM, Chaurio RA, Lopez-Bailon LU, Nagy MZ, Mine JA, Hänggi K, Sprenger KB, Innamarato P, Harro CM, Powers JJ, Johnson J, Fang B, Eysha M, Nan X, Li R, Perez BA, Curiel TJ, Yu X, Rodriguez PC, Conejo-Garcia JR
Immunity 2023 Nov 14;56(11):2570-2583.e6

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25-hydroxycholesterol inhibits classical swine fever virus entry into porcine alveolar macrophages by depleting plasma membrane cholesterol.

Zhang L, Yi Y, Wang T, Song M, Guo K, Zhang Y
Veterinary microbiology 2023 Mar;278:109668

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Oligomeric CHMP7 mediates three-way ER junctions and ER-mitochondria interactions.

Chu Q, Wang J, Du Y, Zhou T, Shi A, Xiong J, Ji WK, Deng L
Cell death and differentiation 2023 Jan;30(1):94-110

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Cholesterol determines the cytosolic entry and seeded aggregation of tau.

Tuck BJ, Miller LVC, Katsinelos T, Smith AE, Wilson EL, Keeling S, Cheng S, Vaysburd MJ, Knox C, Tredgett L, Metzakopian E, James LC, McEwan WA
Cell reports 2022 May 3;39(5):110776

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RAB7 activity is required for the regulation of mitophagy in oocyte meiosis and oocyte quality control during ovarian aging.

Jin X, Wang K, Wang L, Liu W, Zhang C, Qiu Y, Liu W, Zhang H, Zhang D, Yang Z, Wu T, Li J
Autophagy 2022 Mar;18(3):643-660

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Lipid flippase dysfunction as a therapeutic target for endosomal anomalies in Alzheimer's disease.

Kaneshiro N, Komai M, Imaoka R, Ikeda A, Kamikubo Y, Saito T, Saido TC, Tomita T, Hashimoto T, Iwatsubo T, Sakurai T, Uehara T, Takasugi N
iScience 2022 Mar 18;25(3):103869

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Bile acids promote the caveolae-associated entry of swine acute diarrhea syndrome coronavirus in porcine intestinal enteroids.

Yang QY, Yang YL, Tang YX, Qin P, Wang G, Xie JY, Chen SX, Ding C, Huang YW, Zhu SJ
PLoS pathogens 2022 Jun;18(6):e1010620

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Porcine Circovirus Type 3 Enters Into PK15 Cells Through Clathrin- and Dynamin-2-Mediated Endocytosis in a Rab5/Rab7 and pH-Dependent Fashion.

Shi R, Hou L, Wei L, Quan R, Zhou B, Jiang H, Wang J, Zhu S, Song J, Wang D, Liu J
Frontiers in microbiology 2021;12:636307

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Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors.

Wang D, Ye Z, Wei W, Yu J, Huang L, Zhang H, Yue J
eLife 2021 Nov 19;10

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Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor.

Ponsford AH, Ryan TA, Raimondi A, Cocucci E, Wycislo SA, Fröhlich F, Swan LE, Stagi M
Autophagy 2021 Jun;17(6):1500-1518

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Structure-based discovery of neoandrographolide as a novel inhibitor of Rab5 to suppress cancer growth.

Zhang J, Sun Y, Zhong LY, Yu NN, Ouyang L, Fang RD, Wang Y, He QY
Computational and structural biotechnology journal 2020;18:3936-3946

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Interferon-Induced Transmembrane Protein 3 Is a Virus-Associated Protein Which Suppresses Porcine Reproductive and Respiratory Syndrome Virus Replication by Blocking Viral Membrane Fusion.

Zhang A, Duan H, Zhao H, Liao H, Du Y, Li L, Jiang D, Wan B, Wu Y, Ji P, Zhou EM, Zhang G
Journal of virology 2020 Nov 23;94(24)

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Atorvastatin Targets the Islet Mevalonate Pathway to Dysregulate mTOR Signaling and Reduce β-Cell Functional Mass.

Shen L, Gu Y, Qiu Y, Cheng T, Nie A, Cui C, Fu C, Li T, Li X, Fu L, Wang Y, Ni Q, Wang Q, Wang W, Feng B
Diabetes 2020 Jan;69(1):48-59

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The N-glycosylation of Equine Tetherin Affects Antiviral Activity by Regulating Its Subcellular Localization.

Bai B, Wang XF, Zhang M, Na L, Zhang X, Zhang H, Yang Z, Wang X
Viruses 2020 Feb 16;12(2)

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An orally available hypoglycaemic peptide taken up by caveolae transcytosis displays improved hypoglycaemic effects and body weight control in db/db mice.

Lu W, Tian H, Qian P, Li Y, Wang Y, Ge Y, Sai W, Gao X, Yao W
British journal of pharmacology 2020 Aug;177(15):3473-3488

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Tsg101 positively regulates physiologic-like cardiac hypertrophy through FIP3-mediated endosomal recycling of IGF-1R.

Essandoh K, Deng S, Wang X, Jiang M, Mu X, Peng J, Li Y, Peng T, Wagner KU, Rubinstein J, Fan GC
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Jun;33(6):7451-7466

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A crucial role for Arf6 in the response of commissural axons to Slit.

Kinoshita-Kawada M, Hasegawa H, Hongu T, Yanagi S, Kanaho Y, Masai I, Mishima T, Chen X, Tsuboi Y, Rao Y, Yuasa-Kawada J, Wu JY
Development (Cambridge, England) 2019 Feb 4;146(3)

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Rab5a suppresses autophagy to promote drug resistance in cancer cells.

Xu W, Shi Q, Qian X, Zhou B, Xu J, Zhu L, Feng L, Jin H, Wang X
American journal of translational research 2018;10(4):1229-1236

---

Reduced graphene oxide triggered epithelial-mesenchymal transition in A549 cells.

Liao Y, Wang W, Huang X, Sun Y, Tian S, Cai P
Scientific reports 2018 Oct 12;8(1):15188

---

Role of RAB5A in FSHR-mediated signal transduction in human granulosa cells.

Zhu K, Li S, Liu J, Hong Y, Chen ZJ, Du Y
Reproduction (Cambridge, England) 2018 Jun;155(6):505-514

---

Autophagy defects and related genetic variations in renal cell carcinoma with eosinophilic cytoplasmic inclusions.

Yu Z, Ma J, Li X, Liu Y, Li M, Wang L, Zhao M, He H, Zhang Y, Rao Q, Zhao D, Wang Y, Fan L, Li P, Liu Y, Liu F, Zhang F, Ye J, Yan Q, Guo S, Wang Z
Scientific reports 2018 Jul 2;8(1):9972

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Exosomes Exploit the Virus Entry Machinery and Pathway To Transmit Alpha Interferon-Induced Antiviral Activity.

Yao Z, Qiao Y, Li X, Chen J, Ding J, Bai L, Shen F, Shi B, Liu J, Peng L, Li J, Yuan Z
Journal of virology 2018 Dec 15;92(24)

---

Rab5a is overexpressed in oral cancer and promotes invasion through ERK/MMP signaling.

Zhang D, Lu C, Ai H
Molecular medicine reports 2017 Oct;16(4):4569-4576

---

Expression of Rab5a correlates with tumor progression in pancreatic carcinoma.

Li Y, Sun X, Ji D, Kong X, Liu D, Zhao Z, Yan J, Chen S
Virchows Archiv : an international journal of pathology 2017 May;470(5):527-536

---

Identification of TMEM230 mutations in familial Parkinson's disease.

Deng HX, Shi Y, Yang Y, Ahmeti KB, Miller N, Huang C, Cheng L, Zhai H, Deng S, Nuytemans K, Corbett NJ, Kim MJ, Deng H, Tang B, Yang Z, Xu Y, Chan P, Huang B, Gao XP, Song Z, Liu Z, Fecto F, Siddique N, Foroud T, Jankovic J, Ghetti B, Nicholson DA, Krainc D, Melen O, Vance JM, Pericak-Vance MA, Ma YC, Rajput AH, Siddique T
Nature genetics 2016 Jul;48(7):733-9

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MicroRNA-130a inhibits cell proliferation, invasion and migration in human breast cancer by targeting the RAB5A.

Pan Y, Wang R, Zhang F, Chen Y, Lv Q, Long G, Yang K
International journal of clinical and experimental pathology 2015;8(1):384-93

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Semaphorin 3A activates the guanosine triphosphatase Rab5 to promote growth cone collapse and organize callosal axon projections.

Wu KY, He M, Hou QQ, Sheng AL, Yuan L, Liu F, Liu WW, Li G, Jiang XY, Luo ZG
Science signaling 2014 Aug 26;7(340):ra81

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

mouse brain tissue were subjected to SDS PAGE followed by western blot with 11947-1-AP(RAB5A antibody) at dilution of 1:500

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

rat brain tissue were subjected to SDS PAGE followed by western blot with 11947-1-AP( RAB5A Antibody) at dilution of 1:500

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The RAB5A antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human RAB5A. This antibody recognizes human,mouse,rat antigen. The RAB5A antibody has been validated for the following applications: ELISA, IHC, WB analysis.

Appendix

11947-1-AP.pdf

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