Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- F49994 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Rab5 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 182-211 from human Rab5a was used as the immunogen for this Rab5 antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the Rab5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of Rab5 antibody and HL-60 lysate.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Rab5 antibody with paraffin-embedded human brain tissue. Primary antibody was followed by FITC-conjugated goat anti-rabbit lgG. FITC emits green fluorescence. Red counterstaining is PI.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human brain tissue with Rab5 antibody
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS: Rab5 antibody flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
