M01891

antibody from Boster Biological Technology

Targeting: RAB5A RAB5

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M01891

Provider

Boster Biological Technology

Product name

Anti-Rab5 RAB5A Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for RAB5/RAB5A detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC, WB. Reactive species: Human, Mouse, Rat RAB5/RAB5A information: Molecular Weight: 23659 MW; Subcellular Localization: Cell membrane ; Lipid-anchor ; Cytoplasmic side . Early endosome membrane ; Lipid-anchor . Melanosome. Cytoplasmic vesicle . Cell projection, ruffle . Membrane. Cytoplasm, cytosol. Enriched in stage I melanosomes. Alternates between membrane-bound and cytosolic forms.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

EOE-18

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human colon, using Rab5 Antibody(M01891)RAB5A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RAB5A Antibody (M01891)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image