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LearnAB-255-NA
antibody from Novus Biologicals
Targeting: KITLG
DFNA69, FPH2, Kitl, KL-1, MGF, SCF, SF, SLF
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- AB-255-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal SCF/c-kit Ligand Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects human SCF/c-kit Ligand in direct ELISAs and Western blots. In direct ELISA, approximately 45% cross-reactivity with recombinant mouse SCF is observed, approximately 30% cross-reactivity with recombinant canine SCF, recombinant rat SCF, and recombinant feline SCF is observed. It will also neutralize the biological activity of recombinant mouse SCF at a 10 fold higher IgG concentration.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Human peripheral blood eosinophils express stem cell factor.
Human peripheral blood eosinophils express stem cell factor.
Hartman M, Piliponsky AM, Temkin V, Levi-Schaffer F
Blood 2001 Feb 15;97(4):1086-91
Blood 2001 Feb 15;97(4):1086-91
Human peripheral blood eosinophils express stem cell factor.
Hartman M, Piliponsky AM, Temkin V, Levi-Schaffer F
Blood 2001 Feb 15;97(4):1086-91
Blood 2001 Feb 15;97(4):1086-91
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by SCF/c-kit Ligand and Neutralization by Human SCF/c-kit Ligand Antibody. Recombinant Human SCF/c-kit Ligand (Catalog # 255-SC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human SCF/c-kit Ligand (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human SCF/c-kit Ligand Polyclonal Antibody (Catalog # AB-255-NA). The ND50 is typically 0.4-2 µg/mL.
