Explore
Validate
Learn
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB455 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse SCF/c-kit Ligand Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse SCF/c-kit Ligand in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human SCF.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P20826- Isotype
- IgG
- Antibody clone number
- 40215
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
Somatic excision demonstrates that c-Jun induces cellular migration and invasion through induction of stem cell factor.
Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y
The Journal of biological chemistry 2015 Sep 11;290(37):22338-51
The Journal of biological chemistry 2015 Sep 11;290(37):22338-51
Somatic excision demonstrates that c-Jun induces cellular migration and invasion through induction of stem cell factor.
Katiyar S, Jiao X, Wagner E, Lisanti MP, Pestell RG
Molecular and cellular biology 2007 Feb;27(4):1356-69
Molecular and cellular biology 2007 Feb;27(4):1356-69
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by SCF/c-kit Ligand and Neutralization by Mouse SCF/c-kit Ligand Antibody. Recombinant Mouse SCF/c-kit Ligand (Catalog # 455-MC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse SCF/c-kit Ligand (25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse SCF/c-kit Ligand Monoclonal Antibody (Catalog # MAB455). The ND50 is typically 5-30 µg/mL.
