AB-455-NA

antibody from R&D Systems

Targeting: KITLG DFNA69, FPH2, Kitl, KL-1, MGF, SCF, SF, SLF

Western blot (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AB-455-NA

Provider

R&D Systems

Product name

Mouse SCF/c-kit Ligand Antibody

Antibody type

Polyclonal

Description

Protein A or G purified. Detects mouse SCF/c-kit Ligand in direct ELISAs and Western blots. In direct ELISAs, approximately 70% cross-reactivity with recombinant rat SCF/c-kit Ligand is observed, and approximately 25% cross-reactivity with recombinant human SCF/c-kit Ligand and recombinant feline SCF/c-kit Ligand is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q78ED8


Isotype

IgG

Vial size

1 mg

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Adult mouse myometrial label-retaining cells divide in response to gonadotropin stimulation.

Szotek PP, Chang HL, Zhang L, Preffer F, Dombkowski D, Donahoe PK, Teixeira J
Stem cells (Dayton, Ohio) 2007 May;25(5):1317-25

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Cell Proliferation Induced by SCF/c-kit Ligand and Neutralization by Mouse SCF/c-kit Ligand Antibody. Recombinant Mouse SCF/ c-kit Ligand (Catalog # 455-MC) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse SCF/c-kit Ligand (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse SCF/ c-kit Ligand Polyclonal Antibody (Catalog # AB-455-NA). The ND₅₀ is typically 4-20 µg/mL.