Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF-255-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human SCF/c-kit Ligand Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human SCF/c-kit Ligand in direct ELISAs and Western blots. In direct ELISAs, less than 60% cross-reactivity with recombinant mouse SCF is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P21583
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions.
The fibroblast-derived paracrine factor neuregulin-1 has a novel role in regulating the constitutive color and melanocyte function in human skin.
SCF-mediated mast cell infiltration and activation exacerbate the inflammation and immunosuppression in tumor microenvironment.
Human airway epithelial cell determinants of survival and functional phenotype for primary human mast cells.
Growth factor displayed on the surface of retroviral particles without manipulation of envelope proteins is biologically active and can enhance transduction.
Growth differentiation factor-9 and stem cell factor promote primordial follicle formation in the hamster: modulation by follicle-stimulating hormone.
Fibroblasts from human spleen regulate NK cell differentiation from blood CD34(+) progenitors via cell surface IL-15.
Fibroblasts from human spleen regulate NK cell differentiation from blood CD34(+) progenitors via cell surface IL-15.
Human T-cell leukemia virus type 2 induces survival and proliferation of CD34(+) TF-1 cells through activation of STAT1 and STAT5 by secretion of interferon-gamma and granulocyte macrophage-colony-stimulating factor.
Human T-cell leukemia virus type 2 induces survival and proliferation of CD34(+) TF-1 cells through activation of STAT1 and STAT5 by secretion of interferon-gamma and granulocyte macrophage-colony-stimulating factor.
Smith AO, Bowers SL, Stratman AN, Davis GE
PloS one 2013;8(12):e85147
PloS one 2013;8(12):e85147
The fibroblast-derived paracrine factor neuregulin-1 has a novel role in regulating the constitutive color and melanocyte function in human skin.
Choi W, Wolber R, Gerwat W, Mann T, Batzer J, Smuda C, Liu H, Kolbe L, Hearing VJ
Journal of cell science 2010 Sep 15;123(Pt 18):3102-11
Journal of cell science 2010 Sep 15;123(Pt 18):3102-11
SCF-mediated mast cell infiltration and activation exacerbate the inflammation and immunosuppression in tumor microenvironment.
Huang B, Lei Z, Zhang GM, Li D, Song C, Li B, Liu Y, Yuan Y, Unkeless J, Xiong H, Feng ZH
Blood 2008 Aug 15;112(4):1269-79
Blood 2008 Aug 15;112(4):1269-79
Human airway epithelial cell determinants of survival and functional phenotype for primary human mast cells.
Hsieh FH, Sharma P, Gibbons A, Goggans T, Erzurum SC, Haque SJ
Proceedings of the National Academy of Sciences of the United States of America 2005 Oct 4;102(40):14380-5
Proceedings of the National Academy of Sciences of the United States of America 2005 Oct 4;102(40):14380-5
Growth factor displayed on the surface of retroviral particles without manipulation of envelope proteins is biologically active and can enhance transduction.
Chandrashekran A, Gordon MY, Darling D, Farzaneh F, Casimir C
The journal of gene medicine 2004 Nov;6(11):1189-96
The journal of gene medicine 2004 Nov;6(11):1189-96
Growth differentiation factor-9 and stem cell factor promote primordial follicle formation in the hamster: modulation by follicle-stimulating hormone.
Wang J, Roy SK
Biology of reproduction 2004 Mar;70(3):577-85
Biology of reproduction 2004 Mar;70(3):577-85
Fibroblasts from human spleen regulate NK cell differentiation from blood CD34(+) progenitors via cell surface IL-15.
Briard D, Brouty-Boyé D, Azzarone B, Jasmin C
Journal of immunology (Baltimore, Md. : 1950) 2002 May 1;168(9):4326-32
Journal of immunology (Baltimore, Md. : 1950) 2002 May 1;168(9):4326-32
Fibroblasts from human spleen regulate NK cell differentiation from blood CD34(+) progenitors via cell surface IL-15.
Briard D, Brouty-Boyé D, Azzarone B, Jasmin C
Journal of immunology (Baltimore, Md. : 1950) 2002 May 1;168(9):4326-32
Journal of immunology (Baltimore, Md. : 1950) 2002 May 1;168(9):4326-32
Human T-cell leukemia virus type 2 induces survival and proliferation of CD34(+) TF-1 cells through activation of STAT1 and STAT5 by secretion of interferon-gamma and granulocyte macrophage-colony-stimulating factor.
Bovolenta C, Pilotti E, Mauri M, Turci M, Ciancianaini P, Fisicaro P, Bertazzoni U, Poli G, Casoli C
Blood 2002 Jan 1;99(1):224-31
Blood 2002 Jan 1;99(1):224-31
Human T-cell leukemia virus type 2 induces survival and proliferation of CD34(+) TF-1 cells through activation of STAT1 and STAT5 by secretion of interferon-gamma and granulocyte macrophage-colony-stimulating factor.
Bovolenta C, Pilotti E, Mauri M, Turci M, Ciancianaini P, Fisicaro P, Bertazzoni U, Poli G, Casoli C
Blood 2002 Jan 1;99(1):224-31
Blood 2002 Jan 1;99(1):224-31
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by SCF/c-kit Ligand and Neutralization by Human SCF/c-kit Ligand Antibody. Recombinant Human SCF/c-kit Ligand (Catalog # 255-SC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human SCF/c-kit Ligand (10 ng/mL) is neutralized (green line) by increasing concentrations of Human SCF/c-kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-255-NA). The ND50 is typically 0.05-0.3 µg/mL.