Antibody data

Product number
17-9037-41
Provider
Invitrogen Antibodies
Product name
Anti-Phospho-SLP-76 (Tyr128) Monoclonal Antibody (HNDZ55), APC, eBioscience™
Provider product page
Invitrogen Antibodies - 17-9037-41
Antibody type
Monoclonal
Antigen
Other
Description
Description: This HNDZ55 monoclonal antibody recognizes human SH2 domain-containing leukocyte protein of 76 kD (SLP-76) when phosphorylated on tyrosine 128 (Y128). SLP-76 is phosphorylated by ZAP-70 downstream of the T cell receptor (TCR). SLP-76 contains an acidic region that includes a PEST domain, several tyrosine residues that are phosphorylated following TCR ligation, a proline-rich domain, and an SH2 domain. Numerous proteins associate with SLP-76 including Vav1, GADS, and PLC gamma 1, supporting the notion that SLP-76 functions as a critical adaptor protein downstream of the TCR. SLP-76-deficient T cell lines or mice deficient in SLP-76 have shown that SLP-76 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function. This antibody recognizes only human SLP-76 when phosphorylated on Y128. It is not recommended for staining of mouse cells. Applications Reported: This HNDZ55 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This HNDZ55 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Staining Protocol: Protocol A and Protocol C are recommended for this monoclonal antibody. Use of Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins allows for the greatest flexibility for detection of surface and intracellular (cytoplasmic) proteins. Protocol C: Two-step protocol: Fixation/Methanol allows for the greatest discrimination of phospho-specific signaling between unstimulated and stimulated samples, but with limitations on the ability to stain specific surface proteins (refer to "Clone Performance Following Fixation/Permeabilization" located in the Best Protocols Section under the Resources tab online). All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the Best Protocols Section under the Resources tab online. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
HNDZ55
Vial size
25 Tests
Concentration
5 µl/Test
Storage
4° C, store in dark, DO NOT FREEZE!
Provider Type Product Number
- No reagents -