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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [2]
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- Product number
- 17-9037-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-SLP-76 (Tyr128) Monoclonal Antibody (HNDZ55), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This HNDZ55 monoclonal antibody recognizes human SH2 domain-containing leukocyte protein of 76 kD (SLP-76) when phosphorylated on tyrosine 128 (Y128). SLP-76 is phosphorylated by ZAP-70 downstream of the T cell receptor (TCR). SLP-76 contains an acidic region that includes a PEST domain, several tyrosine residues that are phosphorylated following TCR ligation, a proline-rich domain, and an SH2 domain. Numerous proteins associate with SLP-76 including Vav1, GADS, and PLC gamma 1, supporting the notion that SLP-76 functions as a critical adaptor protein downstream of the TCR. SLP-76-deficient T cell lines or mice deficient in SLP-76 have shown that SLP-76 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function.
- Antibody clone number
- HNDZ55
- Concentration
- 5 µL/Test
Submitted references The SLP-76 Src homology 2 domain is required for T cell development and activation.
Conditional deletion of SLP-76 in mature T cells abrogates peripheral immune responses.
Burns JC, Corbo E, Degen J, Gohil M, Anterasian C, Schraven B, Koretzky GA, Kliche S, Jordan MS
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 1;187(9):4459-66
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 1;187(9):4459-66
Conditional deletion of SLP-76 in mature T cells abrogates peripheral immune responses.
Wu GF, Corbo E, Schmidt M, Smith-Garvin JE, Riese MJ, Jordan MS, Laufer TM, Brown EJ, Maltzman JS
European journal of immunology 2011 Jul;41(7):2064-73
European journal of immunology 2011 Jul;41(7):2064-73
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Intracellular staining of normal human peripheral blood cells that were unstimulated (orange histogram), stimulated with Anti-Human CD3 Functional Grade Purified (Product # 16-0037-81) and goat anti-mouse IgG for 5 minutes (purple histogram), or treated with hydrogen peroxide-activated sodium pervanadate (green histogram) with Anti-Human phospho-SLP-76 (Y128) APC. The Intracellular Fixation & Permeabilization Buffer Set (88-8824) and protocol were used for staining. Lymphocytes in the CD3+ gate were used for analysis.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Intracellular staining of normal human peripheral blood cells that were unstimulated (orange histogram), stimulated with Anti-Human CD3 Functional Grade Purified (Product # 16-0037-81) and goat anti-mouse IgG for 5 minutes (purple histogram), or treated with hydrogen peroxide-activated sodium pervanadate (green histogram) with Anti-Human phospho-SLP-76 (Y128) APC. The Intracellular Fixation & Permeabilization Buffer Set (88-8824) and protocol were used for staining. Lymphocytes in the CD3+ gate were used for analysis.
