MA5-28019

antibody from Invitrogen Antibodies

Targeting: LCP2 SLP-76, SLP76

Antibody data

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Validation data

Product number: MA5-28019 - Provider product page
Provider: Invitrogen Antibodies
Product name: Phospho-SLP76 (Tyr128) Recombinant Rabbit Monoclonal Antibody (SLP76Y128-3F8)
Antibody type: Monoclonal
Antigen: Synthetic peptide
Description: Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Antibody clone number: SLP76Y128-3F8
Vial size: 200 μL
Concentration: 0.5 mg/mL
Storage: -20°C

LCP2 protein structure - MA5-28019 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of Phospho-SLP76 in Ramos cells. Sample was incubated with Phospho-SLP76 monoclonal antibody (Product # MA5-28019) at a dilution of 0.01 µg/mL. Cells were treated with secondary antibody only negative control (light blue), untreated (red), treated with pervanadate (green), untreated and blocked with phospho-peptide (black), treated and blocked with phospho peptide (gold), untreated and blocked with non-phospho peptide (dark blue), and treated and blocked with non-phospho peptide (purple).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of Phospho-SLP76 in NIH3T3 cells. Sample was incubated with Phospho-SLP76 monoclonal antibody (Product # MA5-28019) at a dilution of 0.1 µg/mL. Cells were treated with secondary antibody only (blue), isotype control (orange) or primary antibody (green).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of Phospho-SLP76 (Tyr128) using Ramos cells. Samples were incubated in monoclonal Phospho-SLP76 (Tyr128) antibody (Product # MA5-36997) with a dilution of 10 ng/mL. Images correspond with the following conditions: negative control (blue), untreated (grey), treated with pervanadate (orange).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of Phospho-SLP76 (Tyr128) using Ramos cells. Samples were incubated in monoclonal Phospho-SLP76 (Tyr128) antibody (Product # MA5-36997) with a dilution of 10 ng/mL. Images correspond with the following conditions: negative control (blue), untreated (red), treated with pervanadate (green).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of Phospho-SLP76 (Tyr128) using NIH3T3 cells. Samples were incubated in monoclonal Phospho-SLP76 (Tyr128) antibody (Product # MA5-36997) with a dilution of 0.1 µg/mL. Images correspond with the following conditions: secondary antibody (blue), control (orange), primary antibody (green).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of Phospho-SLP76 (Tyr128) using Ramos cells. Samples were incubated in monoclonal Phospho-SLP76 (Tyr128) antibody (Product # MA5-36997) with a dilution of 0.01 µg/mL. Images correspond with the following conditions: negative control (light blue), untreated (red), treated with pervanadate (green), untreated and blocked with phospho-peptide (black), treated and blocked with phospho peptide (gold), untreated and blocked with non-phospho peptide (dark blue), treated and blocked with non-phospho peptide (purple).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of Phospho-SLP76 (Tyr128) using Ramos cells. Samples were incubated in monoclonal Phospho-SLP76 (Tyr128) antibody (Product # MA5-36997) with a dilution of 0.01 µg/mL. Images correspond with the following conditions: negative control (light blue), untreated (red), treated with pervanadate (green), untreated and blocked with phospho-peptide (black), treated and blocked with phospho peptide (gold), untreated and blocked with non-phospho peptide (dark blue), treated and blocked with non-phospho peptide (purple).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of Phospho-SLP76 (Tyr128) using Ramos cells. Samples were incubated in monoclonal Phospho-SLP76 (Tyr128) antibody (Product # MA5-36997) with a dilution of 10 ng/mL. Images correspond with the following conditions: negative control (blue), untreated (grey), treated with pervanadate (orange).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of Phospho-SLP76 (Tyr128) using Ramos cells. Samples were incubated in monoclonal Phospho-SLP76 (Tyr128) antibody (Product # MA5-36997) with a dilution of 10 ng/mL. Images correspond with the following conditions: negative control (blue), untreated (red), treated with pervanadate (green).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of Phospho-SLP76 (Tyr128) using NIH3T3 cells. Samples were incubated in monoclonal Phospho-SLP76 (Tyr128) antibody (Product # MA5-36997) with a dilution of 0.1 µg/mL. Images correspond with the following conditions: secondary antibody (blue), control (orange), primary antibody (green).