Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations [0]
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- Product number
- 625002 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#625002, RRID:AB_2136888
- Product name
- Purified anti-SLP-76
- Antibody type
- Monoclonal
- Antigen
- amino acids 216-434 of human SLP-76
- Description
- SLP-76 is a human Src homology domain-containing leukocyte protein. This cytoplasmic adaptor phosphoprotein contains both SH2 and SAM domains and is involved in B and T cell receptor signaling. The amino terminus of this protein contains three 17 amino acid repeats with conserved tyrosine and acidic residues (DYE(S/P)P) as well as a proline rich region and is known to associate with many proteins involved in T and B cell signaling including Grb2, LAT, Vav1, SLAP-130, SHP-1, and phospholipase C gamma 2. SLP-76 can be phosphorylated on multiple tyrosine residues by the upstream kinases ZAP-70 and Lck. SLP-76 phosphorylation plays an important role in T cell-mediated IL-2 production by allowing phosphorylated Vav to bind; this complex (SLP-76/Vav) stimulates NF-AT and IL-2 gene activation after TCR engagement. Overexpression of SLP-76 has been shown to result in enhanced IL-2 transcription after TCR signaling. The H76 monoclonal antibody recognizes human SLP-76 and has been shown to be useful for Western blotting.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 200 µl
- Storage
- Upon receipt, store frozen at -20°C.
Submitted references The CD3 zeta subunit contains a phosphoinositide-binding motif that is required for the stable accumulation of TCR-CD3 complex at the immunological synapse.
DeFord-Watts LM, Dougall DS, Belkaya S, Johnson BA, Eitson JL, Roybal KT, Barylko B, Albanesi JP, Wülfing C, van Oers NS
Journal of immunology (Baltimore, Md. : 1950) 2011 Jun 15;186(12):6839-47
Journal of immunology (Baltimore, Md. : 1950) 2011 Jun 15;186(12):6839-47
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