sc-25350

antibody from Santa Cruz Biotechnology

Targeting: SOS2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-25350

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-25350, RRID:AB_628270

Product name

Anti-SOS2

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

No bands detected.

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 153

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in a subset of lymphoid cells outside reaction centra.

Validation comment

Staining pattern partly consistent with experimental and/or bioinformatic data.