Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- ELISA [1]
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Validation data
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- Product number
- MAB1920 - Provider product page
- Provider
- R&D Systems
- Product name
- Human SP-D Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human SP-D in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P35247.2
- Isotype
- IgG
- Antibody clone number
- 292201
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Fungal melanin stimulates surfactant protein D-mediated opsonization of and host immune response to Aspergillus fumigatus spores.
Wong SSW, Rani M, Dodagatta-Marri E, Ibrahim-Granet O, Kishore U, Bayry J, Latgé JP, Sahu A, Madan T, Aimanianda V
The Journal of biological chemistry 2018 Mar 30;293(13):4901-4912
The Journal of biological chemistry 2018 Mar 30;293(13):4901-4912
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Human SP-D ELISA Standard Curve. Recombinant Human SP-D protein was serially diluted 2-fold and captured by Mouse Anti-Human SP-D Monoclonal Antibody (Catalog # MAB19201) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human SP-D Monoclonal Antibody (Catalog # MAB1920) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).