MA5-20003

antibody from Invitrogen Antibodies

Targeting: SP3 SPR-2

Antibody data

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Validation data

Product number: MA5-20003 - Provider product page
Provider: Invitrogen Antibodies
Product name: SP3 Monoclonal Antibody (4E5)
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: Peptide Sequence: TAGINADGHL INTGQAMDSS DNSERTGERV SPDINETNTD TDLFVPTSSS SQLPVTIDST GILQQNTNSL TTSSGQVHSS DLQGNYIQSP VSEETQAQNI QVSTAQPVVQ HLQLQESQQP TSQAQIVQGI TPQTIHGVQA SGQN*
Antibody clone number: 4E5
Concentration: 1 mg/mL

SP3 protein structure - MA5-20003 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SP3 in tissue lysate from human stomach cells, using a anti-SP3 monoclonal antibody (Product # MA5-20003).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Transcription factor Sp3 was achieved by transfecting HeLa with Transcription factor Sp3 specific siRNAs (Silencer® select Product # S13324, S13325). Western Blot analysis (Fig. a) was performed using Nuclear enriched extracts from the Transcription factor Sp3 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The Blot was probed with SP3 Monoclonal Antibody (4E5) (Product # MA5-20003, 1:1000 dilution ) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western Blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Transcription factor Sp3.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot was performed using Anti-SP3 Monoclonal Antibody (4E5) (Product # MA5-20003) and a ~85 kDa band corresponding to Transcription factor Sp3 was observed across cell lines tested. Nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1), U-2 OS (Lane 2), HCT 116 (Lane 3), SW480 (Lane 4), HT-29 (Lane 5), NIH/3T3 (Lane 6), PC-12 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).