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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 624801 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#624801, RRID:AB_2194455
- Product name
- Purified anti-Sp3
- Antibody type
- Polyclonal
- Antigen
- Peptide mapping to the carboxy terminus of human Sp3
- Description
- The sequence-specific transcription factor Sp3 binds GC and GT box sequences in the promoters of a variety of genes. The Sp3 transcription factor contains three zinc finger domains that determine sequence specificity of DNA binding. This transcription factor is thought to regulate nucleoside, nucleotide and nucleic acid metabolism and many other "housekeeping" genes. Sp3 can be modified by sumoylation by Ubc9 and has been shown to interact with other transcription factors and histonbe deacetylases. Sp3 has been reported to act as a transcriptional enhancer and repressor of Sp1 activity in a cell- and context-dependent manner. The Poly6248 antibody recognizes the C-terminal region of human, mouse, and rat Sp3 and has been shown to be useful for Western blotting.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 50 µl
- Storage
- The antibody solution should be stored undiluted at -20°C.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Sp3 polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
- Conjugate
- Unconjugated
