MAB2390

antibody from Novus Biologicals

Targeting: THBS4

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB2390

Provider

Novus Biologicals

Product name

Mouse Monoclonal Thrombospondin-4 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Thrombospondin-4 in direct ELISAs and Western blots. Does not cross-react with recombinant human Thrombospondin-1, -2, or -3.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

The clinicopathological significance of Thrombospondin-4 expression in the tumor microenvironment of gastric cancer.

Kuroda K, Yashiro M, Sera T, Yamamoto Y, Kushitani Y, Sugimoto A, Kushiyama S, Nishimura S, Togano S, Okuno T, Tamura T, Toyokawa T, Tanaka H, Muguruma K, Ohira M
PloS one 2019;14(11):e0224727

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Thrombospondin-4 reduces binding affinity of [(3)H]-gabapentin to calcium-channel α2δ-1-subunit but does not interact with α2δ-1 on the cell-surface when co-expressed.

Lana B, Page KM, Kadurin I, Ho S, Nieto-Rostro M, Dolphin AC
Scientific reports 2016 Apr 14;6:24531

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THBS4, a novel stromal molecule of diffuse-type gastric adenocarcinomas, identified by transcriptome-wide expression profiling.

Förster S, Gretschel S, Jöns T, Yashiro M, Kemmner W
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2011 Oct;24(10):1390-403

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Thrombospondin-4 is a putative tumour-suppressor gene in colorectal cancer that exhibits age-related methylation.

Greco SA, Chia J, Inglis KJ, Cozzi SJ, Ramsnes I, Buttenshaw RL, Spring KJ, Boyle GM, Worthley DL, Leggett BA, Whitehall VL
BMC cancer 2010 Sep 16;10:494

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Thrombospondin-4 by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Thrombospondin-4 Monoclonal Antibody (Catalog # MAB2390) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Thrombospondin-4 at approximately 130kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.