MAB2390

antibody from R&D Systems

Targeting: THBS4

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB2390

Provider

R&D Systems

Product name

Human Thrombospondin-4 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Thrombospondin-4 in direct ELISAs and Western blots. Does not cross-react with recombinant human Thrombospondin-1, -2, or -3.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P35443


Isotype

IgG

Antibody clone number

276523

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Thrombospondin-4 reduces binding affinity of [(3)H]-gabapentin to calcium-channel α2δ-1-subunit but does not interact with α2δ-1 on the cell-surface when co-expressed.

Lana B, Page KM, Kadurin I, Ho S, Nieto-Rostro M, Dolphin AC
Scientific reports 2016 Apr 14;6:24531

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THBS4, a novel stromal molecule of diffuse-type gastric adenocarcinomas, identified by transcriptome-wide expression profiling.

Förster S, Gretschel S, Jöns T, Yashiro M, Kemmner W
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2011 Oct;24(10):1390-403

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Thrombospondin-4 is a putative tumour-suppressor gene in colorectal cancer that exhibits age-related methylation.

Greco SA, Chia J, Inglis KJ, Cozzi SJ, Ramsnes I, Buttenshaw RL, Spring KJ, Boyle GM, Worthley DL, Leggett BA, Whitehall VL
BMC cancer 2010 Sep 16;10:494

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Thrombospondin-4 by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Thrombospondin-4 Monoclonal Antibody (Catalog # MAB2390) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Thrombospondin-4 at approximately 130kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.