Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- PA5-34708 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TNFAIP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, HeLa. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.17 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TNFAIP2 using A) A431 and B) HeLa. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a TNFAIP2 polyclonal antibody (Product # PA5-34708) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TNFAIP2 Polyclonal Antibody (Product # PA5-34708). Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with TNFAIP2 Polyclonal Antibody (Product # PA5-34708) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TNFAIP2 Polyclonal Antibody detects TNFAIP2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TNFAIP2 stained by TNFAIP2 Polyclonal Antibody (Product # PA5-34708) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (Product # MA5-31466) diluted at 1:1,000. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TNFalpha-IP 2 antibody [C1C3] detects TNFalpha-IP 2 protein at cytosol on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. TNFalpha-IP 2 antibody [C1C3] (Product # PA5-34708) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TNFalpha-IP 2 antibody [C1C3] detects TNFalpha-IP 2 protein at cytosol on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. TNFalpha-IP 2 antibody [C1C3] (Product # PA5-34708) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TNFalpha-IP 2 antibody [C1C3] detects TNFalpha-IP 2 protein at cytosol on mouse spleen by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. TNFalpha-IP 2 antibody [C1C3] (Product # PA5-34708) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TNFalpha-IP 2 antibody [C1C3] detects TNFalpha-IP 2 protein at cytosol on mouse spleen by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. TNFalpha-IP 2 antibody [C1C3] (Product # PA5-34708) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.