Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- AF6348 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TOP2B Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human TOP2B in Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q02880
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human TOP2B by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 0.2 µg/mL of Sheep Anti-Human TOP2B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6348) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TOP2B at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Western Blot Shows Human TOP2B Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293 human embryonic kidney parental cell line and TOP2B knockout HEK293 cell line (KO). PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human TOP2B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6348) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TOP2B at approximately 245 kDa (as indicated) in the parental HEK293 cell line, but is not detectable in knockout HEK293 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.