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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-34807 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRADD Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: MCF-7, MDA-MB-231.
- Concentration
- 0.85 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TRADD using 30 µg of A431 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a TRADD polyclonal antibody (Product # PA5-34807) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TRADD was performed by loading 30 µg of ME180 (lane1), ME180- TRADD knockout (lane2) whole cell lysate using Novex®NuPAGE®4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (Product # LC5800), and iBlot® Dry Blotting System (Product # IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk for 1 hour at room temperature. TRADD was detected at ~ 34 kDa using TRADD Rabbit Polyclonal Antibody (Product # PA5-34807) at 1:1500 in 5% skim milk at 4°C overnight on a rocking platform. Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106). Loss of signal upon CRISPR mediated knockout (KO) confirms that antibody is specific to TRADD
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of TRADD was performed in paraffin-embedded human endometrial carcinoma tissue using TRADD Polyclonal Antibody (Product # PA5-34807) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
