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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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- Product number
- GTX113029 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113029, RRID:AB_1952377
- Product name
- TRAF6 antibody [N2C2], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Binding and Enhanced Binding between Key Immunity Proteins TRAF6 and TIFA.
The MicroRNAs in the Pathogenesis of Metabolic Memory.
Antiosteoclastogenic activity of silicate-based materials antagonizing receptor activator for nuclear factor kappaB ligand-induced osteoclast differentiation of murine marcophages.
Huang WC, Liao JH, Hsiao TC, Wei TW, Maestre-Reyna M, Bessho Y, Tsai MD
Chembiochem : a European journal of chemical biology 2019 Jan 18;20(2):140-146
Chembiochem : a European journal of chemical biology 2019 Jan 18;20(2):140-146
The MicroRNAs in the Pathogenesis of Metabolic Memory.
Zhong X, Liao Y, Chen L, Liu G, Feng Y, Zeng T, Zhang J
Endocrinology 2015 Sep;156(9):3157-68
Endocrinology 2015 Sep;156(9):3157-68
Antiosteoclastogenic activity of silicate-based materials antagonizing receptor activator for nuclear factor kappaB ligand-induced osteoclast differentiation of murine marcophages.
Hung CJ, Kao CT, Chen YJ, Shie MY, Huang TH
Journal of endodontics 2013 Dec;39(12):1557-61
Journal of endodontics 2013 Dec;39(12):1557-61
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: A549 B: HeLa C: HepG2 7.5% SDS PAGE GTX113029 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: NIH-3T3 B: BCL-1 7.5% SDS PAGE GTX113029 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TRAF6 antibody [N2C2], Internal detects TRAF6 protein by western blot analysis.A. 30 ?g PC-12 whole cell extract B. 30 ?g Rat2 whole cell extract7.5% SDS-PAGETRAF6 antibody [N2C2], Internal (GTX113029) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with TRAF6 antibody (GTX113029) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:2000.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- TRAF6 antibody [N2C2], Internal detects TRAF6 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TRAF6 protein stained by TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:100.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Hoechst 33342 staining.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- TRAF6 antibody [N2C2], Internal immunoprecipitates TRAF6 protein in IP experiments.IP samples: K562 whole cell extractA. Control with 4 £gg of preimmune Rabbit IgGB. Immunoprecipitation of TRAF6 protein by 4 £gg TRAF6 antibody [N2C2], Internal (GTX113029)7.5 % SDS-PAGEThe immunoprecipitated TRAF6 protein was detected by TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded 59T xenograft, using TRAF6(GTX113029) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TRAF6 antibody [N2C2], Internal detects TRAF6 protein at cytoplasm in mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TRAF6 antibody [N2C2], Internal detects TRAF6 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:500.
