- 38-0900 - Invitrogen Antibodies TRAF6 antibody

Submitted references Major vault protein suppresses obesity and atherosclerosis through inhibiting IKK-NF-κB signaling mediated inflammation.

Ben J, Jiang B, Wang D, Liu Q, Zhang Y, Qi Y, Tong X, Chen L, Liu X, Zhang Y, Zhu X, Li X, Zhang H, Bai H, Yang Q, Ma J, Wiemer EAC, Xu Y, Chen Q
Nature communications 2019 Apr 17;10(1):1801

Eicosapentaenoic acid shows anti-inflammatory effect via GPR120 in 3T3-L1 adipocytes and attenuates adipose tissue inflammation in diet-induced obese mice.

Yamada H, Umemoto T, Kakei M, Momomura SI, Kawakami M, Ishikawa SE, Hara K
Nutrition & metabolism 2017;14:33

TRAF6 stimulates the tumor-promoting effects of TGFβ type I receptor through polyubiquitination and activation of presenilin 1.

Gudey SK, Sundar R, Mu Y, Wallenius A, Zang G, Bergh A, Heldin CH, Landström M
Science signaling 2014 Jan 7;7(307):ra2

Cellular inhibitors of apoptosis are global regulators of NF-κB and MAPK activation by members of the TNF family of receptors.

Varfolomeev E, Goncharov T, Maecker H, Zobel K, Kömüves LG, Deshayes K, Vucic D
Science signaling 2012 Mar 20;5(216):ra22

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of (A) HeLa, (B) HEK293, (C) NIH 3T3, and (D) Jurkat cell lysates using Rb anti-TRAF6 (C-term) (Product # 38-0900).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (40 µg lysate) of A431 (Lane 1), A549 (Lane 2), HeLa (Lane 3) and Raji (Lane 4). The blots were probed with Anti-TRAF6 Rabbit Polyclonal Antibody (Product # 38-0900, 1-3 µg/mL) and detected by chemiluminescence Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 60 kDa band corresponding to TRAF6 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of TRAF6 was achieved by transfecting A-431 with TRAF6 specific siRNAs (Silencer® select Product # s14389). Western blot analysis (Fig. a) was performed using whole cell extracts from the TRAF6 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with TRAF6 Polyclonal Antibody (Product # 38-0900, 1 µg/mL) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Absence of signal upon siRNA mediated knock down confirms that antibody is specific to TRAF6.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 7 Protein expression levels of inflammatory cascade and GPR120 in epididymal adipose tissue in the three groups. a Relative protein expression levels of p-IRF3 ( b ), p-JNK ( c ), TRAF6 ( d ), phosphorylated NF-kappaB p65 ( e ) and GPR120 ( f ) in the cytosol or nuclear fraction of epididymal adipose tissue by western blot analysis. Data are presented as the mean +- SEM of three experiments. * P < 0.05 vs. HFHS diet group

38-0900

Antibody data