Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- M00185 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-TRAF6 Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal antibody for TRAF-6/TRAF6 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat TRAF-6/TRAF6 information: Molecular Weight: 59573 MW; Subcellular Localization: Cytoplasm . Cytoplasm, cell cortex . Nucleus . Lipid droplet . Found in the nuclei of some aggressive B-cell lymphoma cell lines as well as in the nuclei of both resting and activated T- and B-lymphocytes. Found in punctate nuclear body protein complexes. Ubiquitination may occur in the cytoplasm and sumoylation in the nucleus. RSAD2/viperin recruits it to the lipid droplet (By similarity); Tissue Specificity: Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Antibody clone number
- BOG-20
- Vial size
- 100ug/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
Submitted references EFHD2 cooperates with E3 ubiquitin ligase Smurf1 to facilitate virus infection by promoting the degradation of TRAF6 in teleost fish.
Sulforaphane inhibits the migration and invasion of BPDE-induced lung adenocarcinoma cells by regulating NLRP12.
Fluoride-induced osteoporosis via interfering with the RANKL/RANK/OPG pathway in ovariectomized rats: Oophorectomy shifted skeletal fluorosis from osteosclerosis to osteoporosis.
The protease calpain2a limits innate immunity by targeting TRAF6 in teleost fish.
Monotropein Protects against Inflammatory Bone Loss and Suppresses Osteoclast Formation and Bone Resorption by Inhibiting NFATc1 via NF-κB and Akt/GSK-3β Pathway.
Jin-Tian-Ge ameliorates ovariectomy-induced bone loss in rats and modulates osteoblastogenesis and osteoclastogenesis in vitro.
eIF3k inhibits NF-κB signaling by targeting MyD88 for ATG5-mediated autophagic degradation in teleost fish.
The synergistic function of miR-140-5p and miR-146a on TLR4-mediated cytokine secretion in osteoarthritic chondrocytes.
IL-33 Can Promote the Process of Pulmonary Fibrosis by Inducing the Imbalance Between MMP-9 and TIMP-1.
Wang P, Li Y, Sun Y, Xu T
Journal of virology 2024 Jan 23;98(1):e0117623
Journal of virology 2024 Jan 23;98(1):e0117623
Sulforaphane inhibits the migration and invasion of BPDE-induced lung adenocarcinoma cells by regulating NLRP12.
Wang W, Chang S, He X, Zhou X, Shang P, Chen Y, Wang X, Chen L, Zhang Q, Qiao Y, Feng F
Toxicology and applied pharmacology 2024 Apr;485:116916
Toxicology and applied pharmacology 2024 Apr;485:116916
Fluoride-induced osteoporosis via interfering with the RANKL/RANK/OPG pathway in ovariectomized rats: Oophorectomy shifted skeletal fluorosis from osteosclerosis to osteoporosis.
Jin Y, Zhou BH, Zhao J, Ommati MM, Wang S, Wang HW
Environmental pollution (Barking, Essex : 1987) 2023 Nov 1;336:122407
Environmental pollution (Barking, Essex : 1987) 2023 Nov 1;336:122407
The protease calpain2a limits innate immunity by targeting TRAF6 in teleost fish.
Chen Y, Wang P, Li Q, Yan X, Xu T
Communications biology 2023 Mar 31;6(1):355
Communications biology 2023 Mar 31;6(1):355
Monotropein Protects against Inflammatory Bone Loss and Suppresses Osteoclast Formation and Bone Resorption by Inhibiting NFATc1 via NF-κB and Akt/GSK-3β Pathway.
Zhang Q, Hu S, He Y, Song Z, Shen Y, Zhao Z, Zhang Q, Qin L, Zhang Q
Nutrients 2022 Sep 24;14(19)
Nutrients 2022 Sep 24;14(19)
Jin-Tian-Ge ameliorates ovariectomy-induced bone loss in rats and modulates osteoblastogenesis and osteoclastogenesis in vitro.
Shen Y, Wang N, Zhang Q, Liu Y, Wu Q, He Y, Wang Y, Wang X, Zhao Q, Zhang Q, Qin L, Zhang Q
Chinese medicine 2022 Oct 5;17(1):78
Chinese medicine 2022 Oct 5;17(1):78
eIF3k inhibits NF-κB signaling by targeting MyD88 for ATG5-mediated autophagic degradation in teleost fish.
Chen Y, Cao B, Zheng W, Sun Y, Xu T
The Journal of biological chemistry 2022 Mar;298(3):101730
The Journal of biological chemistry 2022 Mar;298(3):101730
The synergistic function of miR-140-5p and miR-146a on TLR4-mediated cytokine secretion in osteoarthritic chondrocytes.
Papathanasiou I, Balis C, Trachana V, Mourmoura E, Tsezou A
Biochemical and biophysical research communications 2020 Feb 12;522(3):783-791
Biochemical and biophysical research communications 2020 Feb 12;522(3):783-791
IL-33 Can Promote the Process of Pulmonary Fibrosis by Inducing the Imbalance Between MMP-9 and TIMP-1.
Wu L, Luo Z, Zheng J, Yao P, Yuan Z, Lv X, Zhao J, Wang M
Inflammation 2018 Jun;41(3):878-885
Inflammation 2018 Jun;41(3):878-885
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using TRAF6 Antibody(M00185)TRAF6 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TRAF6 Antibody (M00185)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image