Antibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- A00185 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-TRAF6 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for TRAF6 detection. Tested with WB, IHC-P, FCM, Direct ELISA in Mouse;Rat.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references EFHD2 cooperates with E3 ubiquitin ligase Smurf1 to facilitate virus infection by promoting the degradation of TRAF6 in teleost fish.
Sulforaphane inhibits the migration and invasion of BPDE-induced lung adenocarcinoma cells by regulating NLRP12.
The protease calpain2a limits innate immunity by targeting TRAF6 in teleost fish.
Monotropein Protects against Inflammatory Bone Loss and Suppresses Osteoclast Formation and Bone Resorption by Inhibiting NFATc1 via NF-κB and Akt/GSK-3β Pathway.
Jin-Tian-Ge ameliorates ovariectomy-induced bone loss in rats and modulates osteoblastogenesis and osteoclastogenesis in vitro.
eIF3k inhibits NF-κB signaling by targeting MyD88 for ATG5-mediated autophagic degradation in teleost fish.
microRNA-20-1 and microRNA-101a Suppress the NF-κB-Mediated Inflammation Production by Targeting TRAF6 in Miiuy Croaker.
Zi Qi Decoction Alleviates Liver Fibrosis by Inhibiting the Toll-Like Receptor 4 (TLR4)-Related Nuclear Factor kappa b (NF-κB) and Mitogen-Activated Protein Kinase (MAPK) Signaling Pathways.
LncRNA NEAT1 promotes apoptosis and inflammation in LPS-induced sepsis models by targeting miR-590-3p.
Anti-inflammation effects of injectable platelet-rich fibrin via macrophages and dendritic cells.
Rubiadin-1-methyl ether from Morinda officinalis How. Inhibits osteoclastogenesis through blocking RANKL-induced NF-κB pathway.
Ligusticum wallichii Extract Inhibited the Expression of IL-1β after AMI in Rats.
Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.
Wang P, Li Y, Sun Y, Xu T
Journal of virology 2024 Jan 23;98(1):e0117623
Journal of virology 2024 Jan 23;98(1):e0117623
Sulforaphane inhibits the migration and invasion of BPDE-induced lung adenocarcinoma cells by regulating NLRP12.
Wang W, Chang S, He X, Zhou X, Shang P, Chen Y, Wang X, Chen L, Zhang Q, Qiao Y, Feng F
Toxicology and applied pharmacology 2024 Apr;485:116916
Toxicology and applied pharmacology 2024 Apr;485:116916
The protease calpain2a limits innate immunity by targeting TRAF6 in teleost fish.
Chen Y, Wang P, Li Q, Yan X, Xu T
Communications biology 2023 Mar 31;6(1):355
Communications biology 2023 Mar 31;6(1):355
Monotropein Protects against Inflammatory Bone Loss and Suppresses Osteoclast Formation and Bone Resorption by Inhibiting NFATc1 via NF-κB and Akt/GSK-3β Pathway.
Zhang Q, Hu S, He Y, Song Z, Shen Y, Zhao Z, Zhang Q, Qin L, Zhang Q
Nutrients 2022 Sep 24;14(19)
Nutrients 2022 Sep 24;14(19)
Jin-Tian-Ge ameliorates ovariectomy-induced bone loss in rats and modulates osteoblastogenesis and osteoclastogenesis in vitro.
Shen Y, Wang N, Zhang Q, Liu Y, Wu Q, He Y, Wang Y, Wang X, Zhao Q, Zhang Q, Qin L, Zhang Q
Chinese medicine 2022 Oct 5;17(1):78
Chinese medicine 2022 Oct 5;17(1):78
eIF3k inhibits NF-κB signaling by targeting MyD88 for ATG5-mediated autophagic degradation in teleost fish.
Chen Y, Cao B, Zheng W, Sun Y, Xu T
The Journal of biological chemistry 2022 Mar;298(3):101730
The Journal of biological chemistry 2022 Mar;298(3):101730
microRNA-20-1 and microRNA-101a Suppress the NF-κB-Mediated Inflammation Production by Targeting TRAF6 in Miiuy Croaker.
Cui J, Gu L, Zhong L, Liu X, Sun Y, Xu T
Infection and immunity 2022 Jan 25;90(1):e0058521
Infection and immunity 2022 Jan 25;90(1):e0058521
Zi Qi Decoction Alleviates Liver Fibrosis by Inhibiting the Toll-Like Receptor 4 (TLR4)-Related Nuclear Factor kappa b (NF-κB) and Mitogen-Activated Protein Kinase (MAPK) Signaling Pathways.
Zhou J, Zhang X, Wan L, Yu J, Li T, Lu Z, Fang N, Sun L, Ye F
Medical science monitor : international medical journal of experimental and clinical research 2021 Apr 14;27:e929438
Medical science monitor : international medical journal of experimental and clinical research 2021 Apr 14;27:e929438
LncRNA NEAT1 promotes apoptosis and inflammation in LPS-induced sepsis models by targeting miR-590-3p.
Liu L, Liu F, Sun Z, Peng Z, You T, Yu Z
Experimental and therapeutic medicine 2020 Oct;20(4):3290-3300
Experimental and therapeutic medicine 2020 Oct;20(4):3290-3300
Anti-inflammation effects of injectable platelet-rich fibrin via macrophages and dendritic cells.
Zhang J, Yin C, Zhao Q, Zhao Z, Wang J, Miron RJ, Zhang Y
Journal of biomedical materials research. Part A 2020 Jan;108(1):61-68
Journal of biomedical materials research. Part A 2020 Jan;108(1):61-68
Rubiadin-1-methyl ether from Morinda officinalis How. Inhibits osteoclastogenesis through blocking RANKL-induced NF-κB pathway.
He YQ, Zhang Q, Shen Y, Han T, Zhang QL, Zhang JH, Lin B, Song HT, Hsu HY, Qin LP, Xin HL, Zhang QY
Biochemical and biophysical research communications 2018 Dec 2;506(4):927-931
Biochemical and biophysical research communications 2018 Dec 2;506(4):927-931
Ligusticum wallichii Extract Inhibited the Expression of IL-1β after AMI in Rats.
Yuan Z, Zhang J, Yang C
Evidence-based complementary and alternative medicine : eCAM 2014;2014:620359
Evidence-based complementary and alternative medicine : eCAM 2014;2014:620359
Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.
Liu MW, Wang YH, Qian CY, Li H
International journal of molecular medicine 2014 Dec;34(6):1492-504
International journal of molecular medicine 2014 Dec;34(6):1492-504
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of HEPA 1-6 cells using anti-TRAF6 antibody (A00185). Overlay histogram showing HEPA 1-6 cells stained with A00185 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRAF6 Antibody (A00185, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
- Additional image