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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-121842 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRAF6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 20 µL
- Concentration
- 500 µg/mL
- Storage
- -20°C
Submitted references Sacubitril/valsartan (LCZ696) ameliorates hyperthyroid-induced cardiac hypertrophy in male rats through modulation of miR-377, let-7 b, autophagy, and fibrotic signaling pathways.
Khamis T, Alsemeh AE, Abdullah DM
Scientific reports 2022 Aug 27;12(1):14654
Scientific reports 2022 Aug 27;12(1):14654
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TRAF6 in recombinant human TRAF6. Sample was incubated with polyclonal antibody (Product # PA5-119468).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TRAF6 in Lane 1: Wild-type A431 cell lysate; Lane 2: TRAF6 knockout A431 cell lysate . Samples were incubated with polyclonal antibody (Product # PA5-119468) at a dilution of 5 µg/mL, followed by HRP-Linked Caprine Anti-Rabbit IgG using a dilution of 0.2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TRAF6 in paraffin-embedded Human Cardiac Muscle tissue. Samples were incubated with polyclonal antibody (Product # PA5-119468) at a dilution of 20 µg/mL, followed by HRP-Linked Caprine Anti-Rabbit IgG using a dilution of 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TRAF6 in paraffin-embedded Human Liver tissue. Samples were incubated with polyclonal antibody (Product # PA5-119468) at a dilution of 20 µg/mL, followed by HRP-Linked Caprine Anti-Rabbit IgG using a dilution of 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TRAF6 in paraffin-embedded Human Liver tissue. Samples were incubated with polyclonal antibody (Product # PA5-119468) at a dilution of 20 µg/mL, followed by HRP-Linked Caprine Anti-Rabbit IgG using a dilution of 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- The effect of the LCZ696 and VAL administration on the level of the cardiac inflammatory markers ( A - D ). ( A ) cardiac IL-6 level (Pg/g), ( B ) cardiac IL-1beta level (Pg/g), ( C ) cardiac TNF-alpha (Pg/g) level, ( D ) (i-v) representative photomicrographs showing the estimation of immunohistochemical staining of TRAF-6 in rat cardiac tissues of the different experimental groups (i) Control group, (ii) CH group, (iii) CH + LCZ696 group, (iv) CH + VAL group, and (v) a bar chart demonstrates the changes in the area % of TRAF-6 immune-positive cells of the different experimental groups. Values are mean of 6 rats per group +- S.E.M * p < 0.05; ** p < 0.01; *** p < 0.001. Group means were compared by one-way analysis of variance (ANOVA) followed by Tukey post hoc tests for multiple comparisons.
