Antibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF175 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal TrkA Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human TrkA in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant rat TrkA is observed and 10% cross-reactivity with recombinant human (rh) TrkB and rhTrKC is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Nerve growth factor receptor role on rabbit sperm storage.
In vitro effect of nerve growth factor on the main traits of rabbit sperm.
Prdm12 Directs Nociceptive Sensory Neuron Development by Regulating the Expression of the NGF Receptor TrkA.
Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells.
Nerve growth factor downregulates inflammatory response in human monocytes through TrkA.
Characterization of neuronal populations in the human trigeminal ganglion and their association with latent herpes simplex virus-1 infection.
TrkB antibody elicits cytotoxicity and suppresses migration/invasion of transitional cell carcinoma cells.
TrkB antibody elicits cytotoxicity and suppresses migration/invasion of transitional cell carcinoma cells.
BDNF mediated TrkB activation is a survival signal for transitional cell carcinoma cells.
BDNF mediated TrkB activation is a survival signal for transitional cell carcinoma cells.
Neurotrophin signaling through tropomyosin receptor kinases contributes to survival and proliferation of non-Hodgkin lymphoma.
Castellini C, Mattioli S, Bosco AD, Cotozzolo E, Cartoni Mancinelli A, Rende M, Stabile AM, Pistilli A
Theriogenology 2020 Sep 1;153:54-61
Theriogenology 2020 Sep 1;153:54-61
In vitro effect of nerve growth factor on the main traits of rabbit sperm.
Castellini C, Mattioli S, Dal Bosco A, Collodel G, Pistilli A, Stabile AM, Macchioni L, Mancuso F, Luca G, Rende M
Reproductive biology and endocrinology : RB&E 2019 Nov 12;17(1):93
Reproductive biology and endocrinology : RB&E 2019 Nov 12;17(1):93
Prdm12 Directs Nociceptive Sensory Neuron Development by Regulating the Expression of the NGF Receptor TrkA.
Desiderio S, Vermeiren S, Van Campenhout C, Kricha S, Malki E, Richts S, Fletcher EV, Vanwelden T, Schmidt BZ, Henningfeld KA, Pieler T, Woods CG, Nagy V, Verfaillie C, Bellefroid EJ
Cell reports 2019 Mar 26;26(13):3522-3536.e5
Cell reports 2019 Mar 26;26(13):3522-3536.e5
Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells.
Alshawaf AJ, Viventi S, Qiu W, D'Abaco G, Nayagam B, Erlichster M, Chana G, Everall I, Ivanusic J, Skafidas E, Dottori M
Scientific reports 2018 Jan 12;8(1):603
Scientific reports 2018 Jan 12;8(1):603
Nerve growth factor downregulates inflammatory response in human monocytes through TrkA.
Prencipe G, Minnone G, Strippoli R, De Pasquale L, Petrini S, Caiello I, Manni L, De Benedetti F, Bracci-Laudiero L
Journal of immunology (Baltimore, Md. : 1950) 2014 Apr 1;192(7):3345-54
Journal of immunology (Baltimore, Md. : 1950) 2014 Apr 1;192(7):3345-54
Characterization of neuronal populations in the human trigeminal ganglion and their association with latent herpes simplex virus-1 infection.
Flowerdew SE, Wick D, Himmelein S, Horn AK, Sinicina I, Strupp M, Brandt T, Theil D, Hüfner K
PloS one 2013;8(12):e83603
PloS one 2013;8(12):e83603
TrkB antibody elicits cytotoxicity and suppresses migration/invasion of transitional cell carcinoma cells.
Huang YT, Lai PC, Wu CC, Cheng CC, Chiu TH
International journal of oncology 2010 Oct;37(4):943-9
International journal of oncology 2010 Oct;37(4):943-9
TrkB antibody elicits cytotoxicity and suppresses migration/invasion of transitional cell carcinoma cells.
Huang YT, Lai PC, Wu CC, Cheng CC, Chiu TH
International journal of oncology 2010 Oct;37(4):943-9
International journal of oncology 2010 Oct;37(4):943-9
BDNF mediated TrkB activation is a survival signal for transitional cell carcinoma cells.
Huang YT, Lai PC, Wu CC, Hsu SH, Cheng CC, Lan YF, Chiu TH
International journal of oncology 2010 Jun;36(6):1469-76
International journal of oncology 2010 Jun;36(6):1469-76
BDNF mediated TrkB activation is a survival signal for transitional cell carcinoma cells.
Huang YT, Lai PC, Wu CC, Hsu SH, Cheng CC, Lan YF, Chiu TH
International journal of oncology 2010 Jun;36(6):1469-76
International journal of oncology 2010 Jun;36(6):1469-76
Neurotrophin signaling through tropomyosin receptor kinases contributes to survival and proliferation of non-Hodgkin lymphoma.
Sniderhan LF, Garcia-Bates TM, Burgart M, Bernstein SH, Phipps RP, Maggirwar SB
Experimental hematology 2009 Nov;37(11):1295-309
Experimental hematology 2009 Nov;37(11):1295-309
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human TrkA by Western Blot. Western blot shows lysates of RPMI 8226 human multiple myeloma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TrkA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF175) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TrkA at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- TrkA in Human Colon. TrkA was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human TrkA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF175) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in colon glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by beta-NGF and Neutralization by Human TrkA Antibody. Recombinant Human beta-NGF (Catalog # 256-GF) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human beta-NGF (5 ng/mL) is neutralized (green line) by increasing concent-rations of Goat Anti-Human TrkA Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF175). The ND50 is typically 3-12 µg/mL.