LS-C46129

antibody from LSBio

Targeting: TUBG1 TUBG, TUBGCP1

Western blot (Enhanced validation)

Immunocytochemistry (Recommended by provider)

Antibody Data

Product number

LS-C46129

Provider

LSBio

Product name

TUBG1 / Tubulin Gamma 1 Antibody (aa434-449, clone TU-30) LS-C46129

Antibody type

Monoclonal

Description

Purified by protein-A affinity chromatography. Greater than 95% by SDS-PAGE.

Reactivity

Human, Mouse, Rat, Bovine, Chicken/Avian, Porcine

Host

Mouse

Isotype

IgG

Antibody clone number

TU-30

Storage

Store at 2°C to 8°C. Do not freeze.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Differential reactivity of monoclonal antibodies to ?-tubulin with human ?-tubulin isotypes.  (A) Immunoblots of total cell lysates from SH-SY5Y cells, expressing TagRFP-tagged human ?-tubulin 1 (?-Tb1) or ?-tubulin 2 (?-Tb2), probed with Abs to ?-tubulin (TU-30, TU-32), TagRFP (RFP) and GAPDH. In control samples, only secondary anti-mouse Ab was applied. (B) Immunoblots of immobilized GST-tagged human C-terminal regions (a.a. 362-451) of ?-Tb1 or ?-Tb2 probed with Abs to ?-tubulin (TU-30, TU-32) and GST. In control samples, only secondary anti-mouse Ab was applied.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Differential reactivity of monoclonal antibodies to ?-tubulin with human ?-tubulin isotypes.  (A) Immunoblots of total cell lysates from SH-SY5Y cells, expressing TagRFP-tagged human ?-tubulin 1 (?-Tb1) or ?-tubulin 2 (?-Tb2), probed with Abs to ?-tubulin (TU-30, TU-32), TagRFP (RFP) and GAPDH. In control samples, only secondary anti-mouse Ab was applied. (B) Immunoblots of immobilized GST-tagged human C-terminal regions (a.a. 362-451) of ?-Tb1 or ?-Tb2 probed with Abs to ?-tubulin (TU-30, TU-32) and GST. In control samples, only secondary anti-mouse Ab was applied.