Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- BS1483 - Provider product page
- Provider
- Bioworld Technology, Inc
- Proper citation
- Bioworld Technology Cat#BS1483, RRID:AB_1662992
- Product name
- Tubulin ÎÛ (F414) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide, corresponding to amino acids 380-430 of Human Tubulin ÎÛ.
- Description
- TUB4, the gene for Saccharomyces cerevisiae ÎÛ-Tubulin, encodes a 473 amino acid structural protein that localizes to the spindle pole body. ÎÛ Tubulin, an essential protein for cell growth, organizes microtubule arrays in the nucleus and cytoplasm. ÎÛ Tubulin-depleted cells fail to form functional spindles and arrest during nuclear division. ÎÛ Tubulin associates with spindle body components Spc97 and Spc98 to form the ÎÛ Tubulin complex. The budding yeast ÎÛ Tubulin complex contains one molecule each of Spc97 and Spc98 and two molecules of ÎÛ Tubulin. In the SPB, Spc110 binds Spc97 and Spc98 of the ÎÛ Tubulin complex. 2D gel analysis indicates five isoforms of ÎÛ Tubulin. The phosphorylation of ÎÛ Tubulin at Tyr 445 plays a regulatory role in microtubule formation. The incidence rate for this phosphorylation event peaks during G1.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of Tubulin ¦Ã (F414) pAb at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264.7 whole cell lysateLane3:H9C2 whole cell lysate