PA5-34815

antibody from Invitrogen Antibodies

Targeting: TUBG1 TUBG, TUBGCP1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-34815

Provider

Invitrogen Antibodies

Product name

gamma Tubulin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, Jurkat, Raji, NCI-H929, NIH3T3, rat kidney. Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (98%), Xenopus laevis (99%), Dog (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat, Zebrafish

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.24 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of gamma Tubulin in methanol-fixed 293T cells using a gamma Tubulin polyclonal antibody (Product # PA5-34815) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of gamma Tubulin in paraformaldehyde-fixed 293T cells using a gamma Tubulin polyclonal antibody (Product # PA5-34815) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

gamma Tubulin Polyclonal Antibody detects gamma Tubulin protein at centrosome by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: gamma Tubulin stained by gamma Tubulin Polyclonal Antibody (Product # PA5-34815) diluted at 1:1,000. Red: alpha Tubulin, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:500. Blue: Fluoroshield with DAPI .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

gamma Tubulin Polyclonal Antibody detects gamma Tubulin protein at centrosome by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: gamma Tubulin stained by gamma Tubulin Polyclonal Antibody (Product # PA5-34815) diluted at 1:1,000. Red: alpha Tubulin, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:500. Blue: Fluoroshield with DAPI .

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of gamma Tubulin was performed in Jurkat whole cell extracts using 5 µg of gamma Tubulin Polyclonal Antibody (Product # PA5-34815). Samples were transferred to a membrane and probed with gamma Tubulin Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

gamma Tubulin Polyclonal Antibody detects gamma Tubulin protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Gamma Tubulin Polyclonal Antibody (Product # PA5-34815) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of gamma Tubulin was performed in Jurkat whole cell extracts using 5 µg of gamma Tubulin Polyclonal Antibody (Product # PA5-34815). Samples were transferred to a membrane and probed with gamma Tubulin Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 1 PPP1R2 affects centrosome number through interaction with AURKA and PP1. a-b Schematic of constructs used for transfection and representative transfection. c-i ARPE-19 cells were transfected either singly or in combination with plasmids expressing PPP1R2, AURKA, PP1 and empty vector as control (FLAG). Transfected cells were stained for gamma-tubulin (green) and alpha-tubulin (red) and centrosomes were counted in a minimum of 100 cells for each treatment group in three replicates. Insets magnify cellular regions containing centrosomes. Size bar equals 10 mum. j Graphical representation of the frequency of supernumerary centrosomes in cells transfected with each of the indicated plasmids individually or in combination. Statistically significant differences ( p < 0.05) between groups are indicated by differing letter notations above the bars and error bars represent standard error of the mean. Statistically significant differences are indicated with asterisks (* = p