Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [8]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
- Other assay [2]
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Validation data
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- Product number
- PA5-34815 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- gamma Tubulin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, Jurkat, Raji, NCI-H929, NIH3T3, rat kidney. Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (98%), Xenopus laevis (99%), Dog (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat, Zebrafish
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.24 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of gamma Tubulin using Various whole cell extracts (30 µg). Samples were loaded onto a 10% SDS-PAGE gel and probed with a gamma Tubulin polyclonal antibody (Product # PA5-34815) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of gamma Tubulin using Various whole cell extracts (30 µg). Samples were loaded onto a 10% SDS-PAGE gel and probed with a gamma Tubulin polyclonal antibody (Product # PA5-34815) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of gamma Tubulin was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a gamma Tubulin Polyclonal Antibody (Product # PA5-34815) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of gamma Tubulin was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a gamma Tubulin Polyclonal Antibody (Product # PA5-34815) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using gamma Tubulin Polyclonal Antibody (Product # PA5-34815). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin Polyclonal Antibody (Product # PA5-34815) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- gamma Tubulin Polyclonal Antibody detects gamma Tubulin protein by western blot analysis. NIH3T3 whole cell extracts (30 µg) and rat tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin Polyclonal Antibody (Product # PA5-34815) diluted by 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of gamma Tubulin was achieved by transfecting HeLa cells with gamma Tubulin specific siRNAs (Silencer® select Product # s14502). Western blot analysis (Fig. a) was performed using membrane extracts from the gamma Tubulin knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with gamma Tubulin Polyclonal Antibody (Product # PA5-34815, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to gamma Tubulin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), A549 (Lane 3), Hep G2 (Lane 4), Caco-2 (Lane 5), A-431 (Lane 6), C2C12 (Lane 7), NIH/3T3 (Lane 8) and SH-SY5Y (Lane 9). The blot was probed with Anti-gamma Tubulin Polyclonal Antibody (Product # PA5-34815, 1:2000 dilution) and detected by chemiluminescence using Goat anti Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 51 kDa band corresponding to gamma Tubulin was observed across all cell lines tested. An additional band at 150 kDa was also observed in HeLa, MCF7, A549, Hep G2, A-431, C2C12 and SH-SY5Y.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescent analysis of gamma Tubulin in methanol-fixed 293T cells using a gamma Tubulin polyclonal antibody (Product # PA5-34815) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of gamma Tubulin in paraformaldehyde-fixed 293T cells using a gamma Tubulin polyclonal antibody (Product # PA5-34815) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- gamma Tubulin Polyclonal Antibody detects gamma Tubulin protein at centrosome by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: gamma Tubulin stained by gamma Tubulin Polyclonal Antibody (Product # PA5-34815) diluted at 1:1,000. Red: alpha Tubulin, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:500. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- gamma Tubulin Polyclonal Antibody detects gamma Tubulin protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Gamma Tubulin Polyclonal Antibody (Product # PA5-34815) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of gamma Tubulin was performed in Jurkat whole cell extracts using 5 µg of gamma Tubulin Polyclonal Antibody (Product # PA5-34815). Samples were transferred to a membrane and probed with gamma Tubulin Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1 PPP1R2 affects centrosome number through interaction with AURKA and PP1. a-b Schematic of constructs used for transfection and representative transfection. c-i ARPE-19 cells were transfected either singly or in combination with plasmids expressing PPP1R2, AURKA, PP1 and empty vector as control (FLAG). Transfected cells were stained for gamma-tubulin (green) and alpha-tubulin (red) and centrosomes were counted in a minimum of 100 cells for each treatment group in three replicates. Insets magnify cellular regions containing centrosomes. Size bar equals 10 mum. j Graphical representation of the frequency of supernumerary centrosomes in cells transfected with each of the indicated plasmids individually or in combination. Statistically significant differences ( p < 0.05) between groups are indicated by differing letter notations above the bars and error bars represent standard error of the mean. Statistically significant differences are indicated with asterisks (* = p