- PA5-34815 - Invitrogen Antibodies TUBG1 antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of gamma Tubulin using Various whole cell extracts (30 µg). Samples were loaded onto a 10% SDS-PAGE gel and probed with a gamma Tubulin polyclonal antibody (Product # PA5-34815) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of gamma Tubulin using Various whole cell extracts (30 µg). Samples were loaded onto a 10% SDS-PAGE gel and probed with a gamma Tubulin polyclonal antibody (Product # PA5-34815) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of gamma Tubulin was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a gamma Tubulin Polyclonal Antibody (Product # PA5-34815) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of gamma Tubulin was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a gamma Tubulin Polyclonal Antibody (Product # PA5-34815) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using gamma Tubulin Polyclonal Antibody (Product # PA5-34815). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin Polyclonal Antibody (Product # PA5-34815) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: gamma Tubulin Polyclonal Antibody detects gamma Tubulin protein by western blot analysis. NIH3T3 whole cell extracts (30 µg) and rat tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin Polyclonal Antibody (Product # PA5-34815) diluted by 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Knockdown of gamma Tubulin was achieved by transfecting HeLa cells with gamma Tubulin specific siRNAs (Silencer® select Product # s14502). Western blot analysis (Fig. a) was performed using membrane extracts from the gamma Tubulin knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with gamma Tubulin Polyclonal Antibody (Product # PA5-34815, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to gamma Tubulin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), A549 (Lane 3), Hep G2 (Lane 4), Caco-2 (Lane 5), A-431 (Lane 6), C2C12 (Lane 7), NIH/3T3 (Lane 8) and SH-SY5Y (Lane 9). The blot was probed with Anti-gamma Tubulin Polyclonal Antibody (Product # PA5-34815, 1:2000 dilution) and detected by chemiluminescence using Goat anti Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 51 kDa band corresponding to gamma Tubulin was observed across all cell lines tested. An additional band at 150 kDa was also observed in HeLa, MCF7, A549, Hep G2, A-431, C2C12 and SH-SY5Y.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescent analysis of gamma Tubulin in methanol-fixed 293T cells using a gamma Tubulin polyclonal antibody (Product # PA5-34815) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescent analysis of gamma Tubulin in paraformaldehyde-fixed 293T cells using a gamma Tubulin polyclonal antibody (Product # PA5-34815) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: gamma Tubulin Polyclonal Antibody detects gamma Tubulin protein at centrosome by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: gamma Tubulin stained by gamma Tubulin Polyclonal Antibody (Product # PA5-34815) diluted at 1:1,000. Red: alpha Tubulin, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:500. Blue: Fluoroshield with DAPI .

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: gamma Tubulin Polyclonal Antibody detects gamma Tubulin protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Gamma Tubulin Polyclonal Antibody (Product # PA5-34815) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunoprecipitation of gamma Tubulin was performed in Jurkat whole cell extracts using 5 µg of gamma Tubulin Polyclonal Antibody (Product # PA5-34815). Samples were transferred to a membrane and probed with gamma Tubulin Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 1 PPP1R2 affects centrosome number through interaction with AURKA and PP1. a-b Schematic of constructs used for transfection and representative transfection. c-i ARPE-19 cells were transfected either singly or in combination with plasmids expressing PPP1R2, AURKA, PP1 and empty vector as control (FLAG). Transfected cells were stained for gamma-tubulin (green) and alpha-tubulin (red) and centrosomes were counted in a minimum of 100 cells for each treatment group in three replicates. Insets magnify cellular regions containing centrosomes. Size bar equals 10 mum. j Graphical representation of the frequency of supernumerary centrosomes in cells transfected with each of the indicated plasmids individually or in combination. Statistically significant differences ( p < 0.05) between groups are indicated by differing letter notations above the bars and error bars represent standard error of the mean. Statistically significant differences are indicated with asterisks (* = p

PA5-34815