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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- NB500-574 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-574, RRID:AB_2211115
- Product name
- Mouse Monoclonal gamma Tubulin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. The antibody TU-30 recognizes C-terminal peptide sequence EYHAATRPDYISWGTQ (aa 434-449) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC).
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Porcine
- Host
- Mouse
- Antigen sequence
The epitope was located in the amin
o acid sequence TRPDYI (aa439-444 i
n human), which is present on human
gamma-tubulin 1 but not on human g
amma-tubulin 2.- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Zingerone Suppresses Tumor Development through Decreasing Cyclin D1 Expression and Inducing Mitotic Arrest.
Choi JS, Ryu J, Bae WY, Park A, Nam S, Kim JE, Jeong JW
International journal of molecular sciences 2018 Sep 19;19(9)
International journal of molecular sciences 2018 Sep 19;19(9)
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: gamma Tubulin Antibody (TU-30) [NB500-574] - Western blotting analysis of differential reactivity of monoclonal antibodies to gamma-tubulin with human gamma-tubulin isotypes. (A) Immunoblots of total cell lysates from SH-SY5Y cells, expressing TagRFP-tagged human gamma-tubulin 1 (gamma-Tb1) or gamma-tubulin 2 (gamma-Tb2), probed with Abs to gamma-tubulin (TU-30, TU-32), TagRFP (RFP) and GAPDH. In control samples, only secondary anti-mouse Ab was applied. (B) Immunoblots of immobilized GST-tagged human C-terminal regions (a.a. 362-451) of gamma-Tb1 or gamma-Tb2 probed with Abs to gamma-tubulin (TU-30, TU-32) and GST. In control samples, only secondary anti-mouse Ab was applied.
