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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- MA5-32514 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- gamma Tubulin Recombinant Rabbit Monoclonal Antibody (JF0972)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Hamster, Xenopus
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JF0972
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of gamma Tubulin in different lysates using a Monoclonal antibody (Product #MA5-32514) at a dilution of 1:1,000. Positive control: Lane 1: A431, Lane 2: Jurkat, Lane 3: Mouse brain.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of gamma Tubulin in Hela cells using a gamma Tubulin Monoclonal antibody (Product # MA5-32514) as seen in red. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of gamma Tubulin in MCF-7 cells using a gamma Tubulin Monoclonal antibody (Product # MA5-32514) as seen in red. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of gamma Tubulin in SHG-44 cells using a gamma Tubulin Monoclonal antibody (Product # MA5-32514) as seen in red. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of gamma Tubulin of paraffin-embedded Mouse colon tissue using a gamma-Tubulin Monoclonal antibody (Product #MA5-32514). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of gamma Tubulin of paraffin-embedded Mouse testis tissue using a gamma-Tubulin Monoclonal antibody (Product #MA5-32514). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of gamma Tubulin in Jurkat cells using a gamma Tubulin Monoclonal Antibody (Product # MA5-32514) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
