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Learn15176-1-AP
antibody from Invitrogen Antibodies
Targeting: TUBG1
TUBG, TUBGCP1
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [9]
- Immunohistochemistry [4]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 15176-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- gamma Tubulin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.37 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 15176-1-AP ( gamma Tubulin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 15176-1-AP (gamma Tubulin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF results of gamma tubulin antibody (15176-1-AP, 1:100) with hTERT-RPE cells by Moshe Kim (laboratory of Dr. William S Trimble, University of Toronto). Cells were fixed in ice-cold methanol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MDCK cells using 15176-1-AP (gamma Tubulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF results of gamma tubulin antibody (15176-1-AP, 1:100) with hTERT-RPE cells by Moshe Kim (laboratory of Dr. William S Trimble, University of Toronto). Cells were fixed in ice-cold methanol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF results of gamma tubulin antibody (15176-1-AP, 1:100) with hTERT-RPE cells by Moshe Kim (laboratory of Dr. William S Trimble, University of Toronto). Cells were fixed in ice-cold methanol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MDCK cells using 15176-1-AP (gamma Tubulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF results of gamma tubulin antibody (15176-1-AP, 1:100) with hTERT-RPE cells by Moshe Kim (laboratory of Dr. William S Trimble, University of Toronto). Cells were fixed in ice-cold methanol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (10% Formaldehyde) fixed MDCK cells using 15176-1-AP (gamma Tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF results of gamma tubulin antibody (15176-1-AP, 1:100) with hTERT-RPE cells by Moshe Kim (laboratory of Dr. William S Trimble, University of Toronto). Cells were fixed in ice-cold methanol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MDCK cells using 15176-1-AP (gamma Tubulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate hyperplasia tissue slide using 15176-1-AP (gamma Tubulin antibody at dilution of 1:400 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate hyperplasia tissue slide using 15176-1-AP (gamma Tubulin antibody at dilution of 1:400 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 15176-1-AP ( gamma Tubulin antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 15176-1-AP ( gamma Tubulin antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were intracellularly stained with 0.4 µg Anti-Human Gamma Tubulin (Product # 15176-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (red), or 0.4 µg Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-gamma Tubulin (IP:15176-1-AP, 4ug; Detection:15176-1-AP 1:2000) with HeLa cells lysate 1600ug.
