MA5-24588

antibody from Invitrogen Antibodies

Targeting: SIX1 DFNA23

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24588

Provider

Invitrogen Antibodies

Product name

SIX1 Monoclonal Antibody (CL0185)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: CFKEKSRGVL REWYAHNPYP SPREKRELAE ATGLTTTQVS NWFKNRRQRD RAAEAKEREN TENNNSSSNK QNQLSPLEGG KPLMSSSEEE FSPPQSPDQN SVLLLQGNMG HARSSNYSLP GLTASQPSHG LQTHQHQLQD S

Antibody clone number

CL0185

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SIX1 in Lane1: Marker (kDa), Lane 2: Human cell line RH-30, Lane 3: Human cell line CACO-2. Samples were probed using a SIX1 Monoclonal Antibody (Product # MA5-24588).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SIX1 in Lane1: Marker (kDa), Lane 2: Human cell line RH-30, Lane 3: Human cell line CACO-2. Samples were probed using a SIX1 Monoclonal Antibody (Product # MA5-24588).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts from RH-30 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using SIX1 Monoclonal Antibody (CL0185) (Product # MA5-24588). Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of SIX1 in RH30 cell line using SIX1 Monoclonal Antibody (Product # MA5-24588), showing spotty nuclear staining in green. Microtubule probes are visualized in red (where available).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of SIX1 in human liver tissue shows absence of immunoreactivity (negative control). Samples were probed using a SIX1 Monoclonal Antibody (Product # MA5-24588).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of SIX1 in human striated muscle shows strong nuclear immunoreactivity in the myocytes. Samples were probed using a SIX1 Monoclonal Antibody (Product # MA5-24588).