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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- TA504057 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA504057, RRID:AB_11124589
- Product name
- SIX1 mouse monoclonal antibody, clone OTI1H4 (formerly 1H4)
- Antibody type
- Monoclonal
- Description
- SIX1 mouse monoclonal antibody, clone OTI1H4 (formerly 1H4)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SIX1
- Isotype
- IgG
- Antibody clone number
- OTI1H4
- Vial size
- 100 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIX1 (RC203465, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIX1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-SIX1 monoclonal antibody at 1:200 dilution.
- Validation comment
- WB
