HPA001893

antibody from Atlas Antibodies

Targeting: SIX1 DFNA23

Provider product page for HPA001893

Western blot

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [37]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [1]

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Product number: HPA001893 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA001893, RRID:AB_1079991
Product name: Anti-SIX1
Antibody type: Polyclonal
Description: Polyclonal Antibody against Human SIX1, Gene description: SIX homeobox 1, Alternative Gene Names: DFNA23, Validated applications: WB, IHC, ICC, Uniprot ID: Q15475, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 100 µl
Concentration: 0.4 mg/ml
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Handling: The antibody solution should be gently mixed before use.

SIX1 protein structure - HPA001893 shown in red.

Submitted references AAV‐mediated Gene Cocktails Enhance Supporting Cell Reprogramming and Hair Cell Regeneration

Zhang L, Chen X, Wang X, Zhou Y, Fang Y, Gu X, Zhang Z, Sun Q, Li N, Xu L, Tan F, Chai R, Qi J
Advanced Science 2024;11(29)

Overlapping functions of SIX homeoproteins during embryonic myogenesis

Cox G, Wurmser M, Madani R, Chaverot N, Backer S, Borok M, Dos Santos M, Comai G, Tajbakhsh S, Relaix F, Santolini M, Sambasivan R, Jiang R, Maire P
PLOS Genetics 2023;19(6):e1010781

All-trans retinoic acid changes muscle fiber type via increasing GADD34 dependent on MAPK signal

Adachi Y, Masuda M, Sakakibara I, Uchida T, Niida Y, Mori Y, Kamei Y, Okumura Y, Ohminami H, Ohnishi K, Yamanaka-Okumura H, Nikawa T, Taketani Y
Life Science Alliance 2022;5(7):e202101345

Loss of Elp1 disrupts trigeminal ganglion neurodevelopment in a model of familial dysautonomia

Leonard C, Quiros J, Lefcort F, Taneyhill L
eLife 2022;11

SIX1/EYA1 are novel liver damage biomarkers in chronic hepatitis B and other liver diseases

Xu B, Yang Q, Tang Y, Tan Z, Fu H, Peng J, Xiang X, Gan L, Deng G, Mao Q, Xu P, Jiang Y, Ding J
Annals of Translational Medicine 2021;9(12):992-992

Chromatin Remodelers Interact with Eya1 and Six2 to Target Enhancers to Control Nephron Progenitor Cell Maintenance

Li J, Xu J, Jiang H, Zhang T, Ramakrishnan A, Shen L, Xu P
Journal of the American Society of Nephrology 2021;32(11):2815-2833

Six1 promotes skeletal muscle thyroid hormone response through regulation of the MCT10 transporter

Girgis J, Yang D, Chakroun I, Liu Y, Blais A
Skeletal Muscle 2021;11(1)

Dynamic changes in cis-regulatory occupancy by Six1 and its cooperative interactions with distinct cofactors drive lineage-specific gene expression programs during progressive differentiation of the auditory sensory epithelium

Xu P, Shen L, Ding J, Loh Y, Wong E, Xu J, Fritzsch B, Ramakrishnan A, Zhang T, Li J
Nucleic Acids Research 2020;48(6):2880-2896

Blastula stage specification of avian neural crest

Prasad M, Uribe-Querol E, Marquez J, Vadasz S, Yardley N, Shelar P, Charney R, García-Castro M
Developmental Biology 2020;458(1):64-74

SIX1 cooperates with RUNX1 and SMAD4 in cell fate commitment of Müllerian duct epithelium

Terakawa J, Serna V, Nair D, Sato S, Kawakami K, Radovick S, Maire P, Kurita T
Cell Death & Differentiation 2020;27(12):3307-3320

SIX1 represses senescence and promotes SOX2-mediated cellular plasticity during tumorigenesis

De Lope C, Martín-Alonso S, Auzmendi-Iriarte J, Escudero C, Mulet I, Larrasa-Alonso J, López-Antona I, Matheu A, Palmero I
Scientific Reports 2019;9(1)

BORIS promotes chromatin regulatory interactions in treatment-resistant cancer cells

Debruyne D, Dries R, Sengupta S, Seruggia D, Gao Y, Sharma B, Huang H, Moreau L, McLane M, Day D, Marco E, Chen T, Gray N, Wong K, Orkin S, Yuan G, Young R, George R
Nature 2019;572(7771):676-680

Eya3 partners with PP2A to induce c-Myc stabilization and tumor progression

Zhang L, Zhou H, Li X, Vartuli R, Rowse M, Xing Y, Rudra P, Ghosh D, Zhao R, Ford H
Nature Communications 2018;9(1)

Dynamic transcriptional signature and cell fate analysis reveals plasticity of individual neural plate border cells

Roellig D, Tan-Cabugao J, Esaian S, Bronner M
eLife 2017;6

A Modular Platform for Differentiation of Human PSCs into All Major Ectodermal Lineages

Tchieu J, Zimmer B, Fattahi F, Amin S, Zeltner N, Chen S, Studer L
Cell Stem Cell 2017;21(3):399-410.e7

Respective contribution of the cephalic neural crest and mesoderm to SIX1-expressing head territories in the avian embryo

Fonseca B, Couly G, Dupin E
BMC Developmental Biology 2017;17(1)

Six1 is essential for differentiation and patterning of the mammalian auditory sensory epithelium

Chen P, Zhang T, Xu J, Maire P, Xu P
PLOS Genetics 2017;13(9):e1006967

Increased Six1 expression is associated with poor prognosis in patients with osteosarcoma.

Chao L, Liu J, Zhao D
Oncology letters 2017 May;13(5):2891-2896

Six1 expression is associated with a poor prognosis in patients with glioma.

Zhang X, Xu R
Oncology letters 2017 Mar;13(3):1293-1298

Six1 homeoprotein drives myofiber type IIA specialization in soleus muscle

Sakakibara I, Wurmser M, Dos Santos M, Santolini M, Ducommun S, Davaze R, Guernec A, Sakamoto K, Maire P
Skeletal Muscle 2016;6(1)

SIX1 Oncoprotein as a Biomarker in a Model of Hormonal Carcinogenesis and in Human Endometrial Cancer

Suen A, Jefferson W, Wood C, Padilla-Banks E, Bae-Jump V, Williams C
Molecular Cancer Research 2016;14(9):849-858

SIX1 coordinates with TGFβ signals to induce epithelial-mesenchymal transition in cervical cancer

Sun S, Liu D, Deng Y, Zhang X, Wan D, Xi B, Huang W, Chen Q, Li M, Wang M, Yang F, Shu P, Wu K, Gao Q
Oncology Letters 2016;12(2):1271-1278

The Six1 oncoprotein downregulates p53 via concomitant regulation of RPL26 and microRNA-27a-3p

Towers C, Guarnieri A, Micalizzi D, Harrell J, Gillen A, Kim J, Wang C, Oliphant M, Drasin D, Guney M, Kabos P, Sartorius C, Tan A, Perou C, Espinosa J, Ford H
Nature Communications 2015;6(1)

The homeoprotein SIX1 controls cellular senescence through the regulation of p16INK4A and differentiation-related genes

Adrados I, Larrasa-Alonso J, Galarreta A, López-Antona I, Menéndez C, Abad M, Gil J, Moreno-Bueno G, Palmero I
Oncogene 2015;35(27):3485-3494

Axud1 Integrates Wnt Signaling and Transcriptional Inputs to Drive Neural Crest Formation

Simões-Costa M, Stone M, Bronner M
Developmental Cell 2015;34(5):544-554

3D mouse embryonic stem cell culture for generating inner ear organoids

Koehler K, Hashino E
Nature Protocols 2014;9(6):1229-1244

Overexpression of sineoculis homeobox homolog 1 predicts poor prognosis of hepatocellular carcinoma.

Kong J, Zhou X, Liu S, Jin T, Piao Y, Liu C, Lin Z
International journal of clinical and experimental pathology 2014;7(6):3018-27

Six Homeoproteins and a linc-RNA at the Fast MYH Locus Lock Fast Myofiber Terminal Phenotype

Dawn C, Sakakibara I, Santolini M, Ferry A, Hakim V, Maire P
PLoS Genetics 2014;10(5):e1004386

Persistently Altered Epigenetic Marks in the Mouse Uterus After Neonatal Estrogen Exposure

Jefferson W, Chevalier D, Phelps J, Cantor A, Padilla-Banks E, Newbold R, Archer T, Kinyamu H, Williams C
Molecular Endocrinology 2013;27(10):1666-1677

The miR-106b-25 cluster targets Smad7, activates TGF-β signaling, and induces EMT and tumor initiating cell characteristics downstream of Six1 in human breast cancer

Smith A, Iwanaga R, Drasin D, Micalizzi D, Vartuli R, Tan A, Ford H
Oncogene 2012;31(50):5162-5171

SIX1 promotes epithelial–mesenchymal transition in colorectal cancer through ZEB1 activation

Ono H, Imoto I, Kozaki K, Tsuda H, Matsui T, Kurasawa Y, Muramatsu T, Sugihara K, Inazawa J
Oncogene 2012;31(47):4923-4934

Six1 regulates stem cell repair potential and self-renewal during skeletal muscle regeneration

Le Grand F, Grifone R, Mourikis P, Houbron C, Gigaud C, Pujol J, Maillet M, Pagès G, Rudnicki M, Tajbakhsh S, Maire P
Journal of Cell Biology 2012;198(5):815-832

Expression of Six1 in luminal breast cancers predicts poor prognosis and promotes increases in tumor initiating cells by activation of extracellular signal-regulated kinase and transforming growth factor-beta signaling pathways

Iwanaga R, Wang C, Micalizzi D, Harrell J, Jedlicka P, Sartorius C, Kabos P, Farabaugh S, Bradford A, Ford H
Breast Cancer Research 2012;14(4)

Eya2 is required to mediate the pro-metastatic functions of Six1 via the induction of TGF-β signaling, epithelial–mesenchymal transition, and cancer stem cell properties

Farabaugh S, Micalizzi D, Jedlicka P, Zhao R, Ford H
Oncogene 2011;31(5):552-562

The Six1 homeoprotein induces human mammary carcinoma cells to undergo epithelial-mesenchymal transition and metastasis in mice through increasing TGF-β signaling

Micalizzi D, Christensen K, Jedlicka P, Coletta R, Barón A, Harrell J, Horwitz K, Billheimer D, Heichman K, Welm A, Schiemann W, Ford H
Journal of Clinical Investigation 2009;119(9):2678-2690

Six1 expands the mouse mammary epithelial stem/progenitor cell pool and induces mammary tumors that undergo epithelial-mesenchymal transition

McCoy E, Iwanaga R, Jedlicka P, Abbey N, Chodosh L, Heichman K, Welm A, Ford H
Journal of Clinical Investigation 2009;119(9):2663-2677

Gene expression changes during HPV-mediated carcinogenesis: a comparison between an in vitro cell model and cervical cancer.

Wan F, Miao X, Quraishi I, Kennedy V, Creek KE, Pirisi L
International journal of cancer 2008 Jul 1;123(1):32-40

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Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western blot analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SIX1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Sample type: Human
Protocol: Protocol

Supportive validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Immunohistochemistry analysis in human skeletal muscle and liver tissues using HPA001893 antibody. Corresponding SIX1 RNA-seq data are presented for the same tissues.
Sample type: Human
Protocol: Protocol
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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